Protein Info for Dshi_3420 in Dinoroseobacter shibae DFL-12

Updated annotation (from data): Glycerol kinase (EC 2.7.1.30)
Rationale: Specifically important for utilizing Glycerol. Automated validation from mutant phenotype: the predicted function (GLYCEROL-KIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: glycerol kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 TIGR01311: glycerol kinase" amino acids 26 to 517 (492 residues), 672.4 bits, see alignment E=1.5e-206 PF00370: FGGY_N" amino acids 27 to 272 (246 residues), 245.4 bits, see alignment E=6.8e-77 PF02782: FGGY_C" amino acids 283 to 471 (189 residues), 137.4 bits, see alignment E=5.4e-44

Best Hits

Swiss-Prot: 76% identical to GLPK_ROSDO: Glycerol kinase (glpK) from Roseobacter denitrificans (strain ATCC 33942 / OCh 114)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to dsh:Dshi_3420)

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LP88 at UniProt or InterPro

Protein Sequence (519 amino acids)

>Dshi_3420 Glycerol kinase (EC 2.7.1.30) (Dinoroseobacter shibae DFL-12)
MDRQRGQFLLNLRALKPRPGEGTVRHVLAIDQGTTSSRAIVFDADMNIVSIAQEEFAQHY
PDSGWVEHDPEDLWDTVLRTCRNVLERTGLAATDLAGIGITNQRETTVVWDKSTGKPIHN
AIVWQDRRTAPFCAELRKAGHDALITAQTGLLADPYFSGTKLKYILDTVEGARDRAKAGE
LLFGTVDSFLIWRLTNGAAHVTDATNAARTMLYDIHKGAWSAEICTLFDIPLGMLPQVHD
CDAEFGTCTPEHLGGAVPILGVAGDQQAATIGQACFEPGMLKSTYGTGCFALLNTGEAPV
TSTNRLLTTIAYQLDGKPTYALEGSIFVAGAVVQWLRDGLKLIANASETQPLAEAADPHD
PVILVPAFTGLGAPYWNAECRGAVFGLSRGSGPEEFARAALESVGYQTRDLLEAMHKDWS
DAREGQPTLRVDGGMTASDWTMQFLADIIDAPVDRPKITETTALGVAWLAGQKAGLYPDR
AGFAANWALDQRFEPKMDATTRDTKYAAWKRAVAAVQQA