Protein Info for Dshi_3373 in Dinoroseobacter shibae DFL-12

Annotation: chromosomal replication initiator protein DnaA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 PF11638: DnaA_N" amino acids 4 to 64 (61 residues), 63.9 bits, see alignment E=2.3e-21 TIGR00362: chromosomal replication initiator protein DnaA" amino acids 6 to 458 (453 residues), 533.1 bits, see alignment E=3.1e-164 PF00308: Bac_DnaA" amino acids 120 to 281 (162 residues), 223.3 bits, see alignment E=4.9e-70 PF01695: IstB_IS21" amino acids 156 to 255 (100 residues), 33.2 bits, see alignment E=1e-11 PF00004: AAA" amino acids 157 to 254 (98 residues), 26.8 bits, see alignment E=1.6e-09 PF08299: Bac_DnaA_C" amino acids 369 to 437 (69 residues), 106.7 bits, see alignment E=1.2e-34

Best Hits

KEGG orthology group: K02313, chromosomal replication initiator protein (inferred from 100% identity to dsh:Dshi_3373)

Predicted SEED Role

"Chromosomal replication initiator protein DnaA" in subsystem DNA-replication

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LNL2 at UniProt or InterPro

Protein Sequence (460 amino acids)

>Dshi_3373 chromosomal replication initiator protein DnaA (RefSeq) (Dinoroseobacter shibae DFL-12)
MTEDTWGSVQNDLLKTVGQNNYLTWIEPLKFADCRGGVVRFEAPSSFVGNWVARNYREDI
LHHLVSAGVEVKRIEFDVAKLAQAARQVRPKGTERTMTPSPGDVAMDKPVPSASLQSAPL
DPRLTFSNFVVGKPNELAHASARRVAEGGPVSFNPLFLYGGVGLGKTHLMHAIAWEAQAR
NPGLKVLYLSAEQFMYRFVRALRDRNTMEFKELFRSVDLLMVDDVQFIAGKDSTQDEFFH
TFNALVDQNKQIIISADRAPGEIDGLEDRIKSRLSCGLVVDIHPTDYELRLGVLQQRLQD
QIAAGETIAVEDGVLECLAQRISTNVRVLEGALNRLIAFSDLVGRRITLDLAHEALSDLI
RAHDKKVTVDEIMQAVAKYYNLRMTDLLGPRRTRTIARPRQLAMYLAKTLTTRSLPEIGR
RFGGRDHTTVIHAVKRIEALCDSDPQISEDVRSLLRTLEN