Protein Info for Dshi_3318 in Dinoroseobacter shibae DFL-12
Annotation: D-3-phosphoglycerate dehydrogenase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 44% identical to SERA_SYNY3: D-3-phosphoglycerate dehydrogenase (serA) from Synechocystis sp. (strain PCC 6803 / Kazusa)
KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 100% identity to dsh:Dshi_3318)MetaCyc: 44% identical to glycerate dehydrogenase (Synechocystis sp. PCC 6803)
Glycerate dehydrogenase. [EC: 1.1.1.29]
Predicted SEED Role
"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)
MetaCyc Pathways
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
- L-serine biosynthesis I (3/3 steps found)
- photorespiration I (7/9 steps found)
- photorespiration III (7/9 steps found)
- L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
- photorespiration II (7/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.95
Use Curated BLAST to search for 1.1.1.29 or 1.1.1.95
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LNF7 at UniProt or InterPro
Protein Sequence (531 amino acids)
>Dshi_3318 D-3-phosphoglycerate dehydrogenase (RefSeq) (Dinoroseobacter shibae DFL-12) MAPKVLVSDKLSETAVQIFRDRGIEVDFEPSLGKDKDKLAEVIGKYDGLAIRSATKVTEK LLAHADNLKVVGRAGIGTDNVDKVAASKKGVIVMNTPFGNMITTAEHAIAMMFAVARQIP EASASTHAGKWEKSKFMGVELTAKTLGVIGAGNIGGIVCERALGLKMKVIAYDPFLSEER ADKLGVEKVDLDTLLARADFITLHVPLTDQTRNILSREALEKTKKGVRIINCARGGLVDE QALADLLKSGHVAGAAFDVFAEEPATENPLFGLPNVVCTPHLGASTTEAQENVALQVAEQ MSNYLLSGAVENALNMPSVTAEEAKTMGPWVKLAGHLGTFVGQLTDEPIDSIEILYNGTV AKMNLEALNCAVIAGIMQATNPDVNMVSAPVIAKERGIGLSTTTQDKSGVFDGYVKLTVK TDQRTRSIAGTVFSDGKPRFIQIKGINIDAEIGEHMLYTTNKDTPGMIGTLGGILGENQV NIANFTLGRNETNGDAIAMLYLDAPLPRDVQDKLIATGLFQQVRPLQFNVA