Protein Info for Dshi_3294 in Dinoroseobacter shibae DFL-12
Annotation: deoxyxylulose-5-phosphate synthase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to DXS2_ROSDO: 1-deoxy-D-xylulose-5-phosphate synthase 2 (dxs2) from Roseobacter denitrificans (strain ATCC 33942 / OCh 114)
KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 100% identity to dsh:Dshi_3294)MetaCyc: 54% identical to 1-deoxy-D-xylulose 5-phosphate synthase (Arabidopsis thaliana col)
1-deoxy-D-xylulose-5-phosphate synthase. [EC: 2.2.1.7]
Predicted SEED Role
"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)
MetaCyc Pathways
- superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (12/12 steps found)
- taxadiene biosynthesis (engineered) (12/13 steps found)
- methylerythritol phosphate pathway I (9/9 steps found)
- methylerythritol phosphate pathway II (9/9 steps found)
- isoprene biosynthesis I (9/10 steps found)
- pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
- superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
- superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (3/6 steps found)
- superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (7/12 steps found)
- superpathway of ergosterol biosynthesis II (11/26 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Terpenoid biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.2.1.7
Use Curated BLAST to search for 2.2.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LMV2 at UniProt or InterPro
Protein Sequence (643 amino acids)
>Dshi_3294 deoxyxylulose-5-phosphate synthase (RefSeq) (Dinoroseobacter shibae DFL-12) MSDRPHTPVLDRVKTPDDMKGLSDRELVALARDLRAETISAVSVTGGHLGAGLGVVELTV ALHAVFDAPRDRIIWDVSHQCYPHKILTGRRDRIRTLRMKDGLSGFTKRSESPYDPFGAA HSSTSISAALGFAVARDLGGAPEHGIGDAIAVIGDGAISAGMAFEALNNAGHLKKRLIVI LNDNEMSIAPPTGAMSSYLNRLYAGAPFQEFKAAAKGAVSLLPPPFQEGAKRAKDILKHM TIGGTLFEELGFSYVGPIDGHDMEQLLSVLRTVKARADGPILIHAITKKGKGYAPAEGAA DKGHATAKFDVVSGAQKKAPSNAPTYTNVFAETLIDEAERDSRIVAVTAAMPSGTGLDKF AKRFAARTFDVAIAEQHGVTFCAGLAAGGMKPFCTMYSTFLQRGYDQVVHDVALQNLPVR FAIDRAGLVGADGATHAGAYDTAYLSNLPNMVVMAAADEAELRHMVATAVAHDSGPIAFR FPRGEGVGVELPERGVPLEIGKGRIIAEGSRVAILSFGTRLAEVREACEALTARGITPTV ADARFAKPLDRDLILRLAAEHEALITIEEGAVGGFGSHVAQLLSEEGVFDTGLKFRSMVL PDTFIDQASPRDMYDIAGLNAPDIEAKVLSVLGVAQIDRRAQS