Protein Info for Dshi_3123 in Dinoroseobacter shibae DFL-12

Updated annotation (from data): Tricarboxylate transport protein TctC
Rationale: specific phenotype on citrate and cofit with other nearby components; SEED_correct
Original annotation: hypothetical protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF03401: TctC" amino acids 70 to 324 (255 residues), 87.2 bits, see alignment E=7.4e-29

Best Hits

KEGG orthology group: K07795, putative tricarboxylic transport membrane protein (inferred from 100% identity to dsh:Dshi_3123)

Predicted SEED Role

"Tricarboxylate transport protein TctC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLA7 at UniProt or InterPro

Protein Sequence (326 amino acids)

>Dshi_3123 Tricarboxylate transport protein TctC (Dinoroseobacter shibae DFL-12)
MTLEFTRRTLIAAAAALAMTGGAHAEGEQMLESIHFLIPGGAGGGWDGTARGTGEALTKA
GLVGSASYENMSGGGGGKAIAYLIENANSSHGTLMVNSTPIVIRSLTGEISQSFRDLTLV
AGTIGDYAAIVVGKDSPINSMADLIAAYDADPNATAVGGGSVPGGMDHLVAAMVMEAAGK
DALGVKYIPYDAGGKAMAALLSGEIAALSTGFSEAIDLAEAGEVKIIGVTAPERVAAYDS
APTMVEQGIDTTFVNWRGFFAAPGLPEEQLAAYQATLEKMYDTPEWEEVRARNGWVNIHN
SGADFQSFLEAQEAQIGDLMKKLGFL