Protein Info for Dshi_3106 in Dinoroseobacter shibae DFL-12

Annotation: pseudouridine synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 24 to 171 (148 residues), 108.1 bits, E=2.4e-35

Best Hits

Swiss-Prot: 56% identical to RLUA_YERPE: Ribosomal large subunit pseudouridine synthase A (rluA) from Yersinia pestis

KEGG orthology group: K06177, ribosomal large subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to dsh:Dshi_3106)

MetaCyc: 56% identical to 23S rRNA pseudouridine746 and tRNA pseudouridine32 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11843 [EC: 5.4.99.29]; RXN-11842 [EC: 5.4.99.29, 5.4.99.28]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.28 or 5.4.99.29

Sequence Analysis Tools

See A8LL90 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Dshi_3106 pseudouridine synthase (RefSeq) (Dinoroseobacter shibae DFL-12)
MPPIPPDTYAPPQEPLRLVHEDAHLLLVEKPSGLLSVPGRGPELADCLLSRVQGAFPDAL
LVHRLDRDTSGLMVFALTAHAQRHLGLQFEKRQIKKSYVARVWGQVAEATGTVDLPLIVD
WPNRPLQKVDPETGKAAVTDWRVLKRSEAETRVRLFPKTGRSHQLRVHMQALGHPILGDP
FYAEGAARAFPRLMLHSESLRLRHPDGGVGMTFRSKPPF