Protein Info for Dshi_2748 in Dinoroseobacter shibae DFL-12

Annotation: glutamyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 3 to 302 (300 residues), 248.9 bits, E=6.1e-78
• TIGR00464: glutamate--tRNA ligase
  • amino acids 3 to 322 (320 residues), 315.4 bits, E=3.6e-98
• PF19269: Anticodon_2
  • amino acids 386 to 440 (55 residues), 25.3 bits, 1.8e-09

Best Hits

Swiss-Prot: 100% identical to SYE2_DINSH: Glutamate--tRNA ligase 2 (gltX2) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 100% identity to dsh:Dshi_2748)

Predicted SEED Role

"Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.24)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (25/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (27/36 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• superpathway of heme b biosynthesis from glutamate (7/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

See A8LIP0 at UniProt or InterPro

Protein Sequence (440 amino acids)

>Dshi_2748 glutamyl-tRNA synthetase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTTTRFAPSPTGYLHVGNLRTALFNYMIARKAGGTFILRIDDTDPERSKPEYVDAIQEDL
TWLGLTWDRIEYQSRRLDRYAEAADALRAAGRFYEAFETPTELDLKRKKQLNMGRPPVYD
RAALALSEDEKAALRAERGQGVWRFKLDHERITWTDGILGDISIDAASVSDPVLIRGDGQ
VLYTIASVVDDTEMGVTHVVRGSDHVTNTATQIQIMAALGHGHPEFAHHSLLTGPQGEAL
SKRLGTLALRDLRAEGIEPMALLSLMARLGSSQPVEVAGSLEALIEGFDLSTFGAAPTKF
DRADLFPLTARLLHATPFAEVADEIAALGVPAAQAELFWEVARANITTRADLAGWWQLFR
DGGAPLVAEEDRAFVADAFARLPEPPYGPETWGAWTAEVKAASGRKGKGLFMPLRKAVTG
LERGPEMADVMRLLQKKPTL