Protein Info for Dshi_2680 in Dinoroseobacter shibae DFL-12

Annotation: glycine dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 954 PF02347: GDC-P" amino acids 16 to 439 (424 residues), 584.2 bits, see alignment E=1.7e-179 amino acids 455 to 727 (273 residues), 48.6 bits, see alignment E=5.7e-17 TIGR00461: glycine dehydrogenase" amino acids 17 to 943 (927 residues), 1418.6 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 772 to 893 (122 residues), 192.3 bits, see alignment E=2.3e-61

Best Hits

Swiss-Prot: 74% identical to GCSP_AGRVS: Glycine dehydrogenase (decarboxylating) (gcvP) from Agrobacterium vitis (strain S4 / ATCC BAA-846)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 74% identity to avi:Avi_2252)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LIH2 at UniProt or InterPro

Protein Sequence (954 amino acids)

>Dshi_2680 glycine dehydrogenase (RefSeq) (Dinoroseobacter shibae DFL-12)
MGFSLTDYAPYDFANRRHIGPSPKEMGQMLATLGVPSLEALINEALPEGIRRRDPLAFGP
ALSERDTLHRMRELADKNTVLTSLIGQGYHGTHTPPVILRNILENPAWYTAYTPYQPEIS
QGRLEALLNFQTMMADLTGLPIANASLLDEGTAAAEAMAMAQRASKSKARGFFVAEDCHP
QTIDVIRTRAEPLGIEVIVGAVDALDPEAVFAALFQYPGSYGHVRDYSDVIEALHGARAL
AVVAADPLALTLLKSPGEMGADIAIGSTQRFGVPMGYGGPHAAYMTCTDALKRSMPGRII
GVSVDARGNKAYRLSLQTREQHIRREKANSNVCTAQALLAVMASMYGVFHGPDGLKAIAQ
TVHRKTARMADGLTELGFKVDPQDYFDTITVKVGSMQGVILAAALREGVNLRKVGTDRIG
ITLDELTLGRTIEAVWRAFGAEGMVYDKTRMVYHLPQEMLRESSYMTHPIFHMNRAEAEM
TRYMRRLADRDLALDRAMIPLGSCTMKLNATVEMLPLTWPEFANLHPFAPADQAAGYHEM
IAELSQMLCDVTGYDAMSMQPNSGAQGEYAGLLAIRGYHRARGEGHRNICLIPTSAHGTN
PASAQMVGWKVVVVKSAENGDIDLEDFRAKAEQHSENLAGCMITYPSTHGVFEEIVREVC
DITHAHGGQVYIDGANMNAMVGLSAPGDLGGDVSHLNLHKTFCIPHGGGGPGMGPIGVKA
HLAPHLPSHATATGAGFGDAGAVASAAYGSPSILTISYAYCLLMGGAGLTQATKVAILNA
NYMAKRLSAGFPILYANDKGRVAHECILDTRVLDKIAGVTVEDVAKRLMDCGFHAPTMSW
PVAGTLMVEPTESEPKAELDRFCDAMLAIREEADAIAAGSLDAENNPLKRAPHTVEDLVG
DWDRPYSREQACYPPGAFRVDKYWPPVNRVDNAYGDRNLVCTCPPVEDYAEPAE