Protein Info for Dshi_2639 in Dinoroseobacter shibae DFL-12

Annotation: carbamoyl-phosphate synthase, large subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1090 (1089 residues), 1459.2 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 7 to 124 (118 residues), 157.1 bits, E=5.9e-50
  • amino acids 569 to 684 (116 residues), 159.3 bits, E=1.2e-50
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 226.8 bits, E=7.6e-71
  • amino acids 688 to 889 (202 residues), 105.1 bits, E=1.3e-33
• PF02787: CPSase_L_D3
  • amino acids 433 to 511 (79 residues), 83.5 bits, 3e-27
• PF02222: ATP-grasp
  • amino acids 696 to 860 (165 residues), 27.4 bits, E=8.5e-10
• PF02655: ATP-grasp_3
  • amino acids 700 to 860 (161 residues), 28.5 bits, E=5.4e-10
• PF02142: MGS
  • amino acids 993 to 1078 (86 residues), 69.6 bits, 7.3e-23

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• allantoin degradation IV (anaerobic) (5/9 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See A8LI38 at UniProt or InterPro

Protein Sequence (1105 amino acids)

>Dshi_2639 carbamoyl-phosphate synthase, large subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MPKRTDIKSIMIIGAGPIIIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPGL
ADATYIEPITPEIVAKIIEKERPDALLPTMGGQTGLNTSLALEEMGVLAKYGVEMIGAKR
EAIEMAEDRKLFREAMDRLGIENPRATIATTMDECMAALDDIGLPAIIRPAFTLGGTGGG
VAYNRDDYEHFCKSGLDASPVNQILIDESLLGWKEFEMEVVRDKADNAIIVCAIENVDPM
GVHTGDSITVAPALTLTDKEYQIMRNGSIAVLREIGVETGGSNVQWAVNPADGRMVVIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELDNDITKVTPASFEPTIDYVVTKIPR
FAFEKFPGAEPNLTTAMKSVGEAMSIGRTFHESVQKALASMETGLTGFDEIAIPGISADH
RSDAPDTAAVVKALARQTPDRLRVIAQAMRHGLSDDEIQAATSYDPWFLARIREIVETEA
QVRRDGLPLEAEGLRKLKMMGFTDARLAKLTGRDEGQVRRARTRLGVTAQFKRIDTCAAE
FEAQTPYMYSTYETPVMGEAECESRPTDATKVVILGGGPNRIGQGIEFDYCCCHACFALT
EAGYETIMVNCNPETVSTDYDTSDRLYFEPLTFEHVMEILRAEQENGTLHGVIVQFGGQT
PLKLANALEAEGIPILGTTPDAIDLAEDRERFQALVNDLGLKQPHNAIASTDAEAFAAAG
DIGFPLVIRPSYVLGGRAMEIVRDMGQLERYIAEAVVVSGDSPVLLDSYLAGAVELDVDA
LCDGENVHVAGIMQHIEEAGVHSGDSACSLPPYSLSDDVLARIRVQTEALARALRVKGLM
NVQFAIKDDEIYLIEVNPRASRTVPFVAKATDSAIASIAARLMAGEPLSNFPLRDPLPHD
APEDQHLPIGDPMTLAHPDTPWFSVKEAVLPFARFPGVDTILGPEMRSTGEVMGWDRSFP
RAFLKAQMGAGTVLPTEGTVFLSIKEADKTEMLVETAAMLTELGLDIVATRGTAAFLKDH
GIASKVVNKVYEGRPDVVDMLKDGRIALVMNTTEGAQAVNDSREIRSVALYDRIPYFTTL
AASHAAAQAMIARREGEIGVRALQG