Protein Info for Dshi_2589 in Dinoroseobacter shibae DFL-12

Annotation: ATP dependent DNA ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 2 to 530 (529 residues), 668.5 bits, see alignment E=4.4e-205 PF04675: DNA_ligase_A_N" amino acids 2 to 160 (159 residues), 47.4 bits, see alignment E=3.9e-16 PF01068: DNA_ligase_A_M" amino acids 221 to 405 (185 residues), 105.6 bits, see alignment E=4.5e-34 PF04679: DNA_ligase_A_C" amino acids 426 to 515 (90 residues), 48.3 bits, see alignment E=1.6e-16

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 100% identity to dsh:Dshi_2589)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LHY8 at UniProt or InterPro

Protein Sequence (534 amino acids)

>Dshi_2589 ATP dependent DNA ligase (RefSeq) (Dinoroseobacter shibae DFL-12)
MKDFAALFTSLDQTTKITPKVAALAAYFRTAPEEDRLWTIALLSGRRPKRTVTTTRLREW
AAERAGIPLWLFEEAYPVVGDLAETIALVLPPPTRTSTRSLTDWITTIRGLAARDEPDRR
AEILAAWDSLPETERFVFNKLITGGFRMGVSAKLMTRALSQATDIPEPELAHRLMGDWTP
ETTSFQSLVLTRDPAADLSKPYPFYLAYGLEDPPDTLGPPADWQAEHKWDGIRGQLILRG
GQHFVWSRGEELMTDRFPELAALRDFLPDGTVLDGELLAWDYNGAGDAPLPFNALQKRIG
RKTVPKKLLSEAPVILRAYDLLEDGGTDLRDRPLSERRARLEALAAALPDTLPLRLSPTL
TFDSWDALAQIRAKSREMGAEGIMLKRRASPYLAGRKKGDWWKWKVDPLTIDAVMIYAQA
GHGRRANLFTDYTFAVWDGNELVPFTKAYSGLTDAEFRQITAWVRKNTLERFGPVRRVTP
EHVFEIAFEGIQASPRHKSGVALRFPRMLRWRKDKPLQEANTLEDLKGMLEVYG