Protein Info for Dshi_2580 in Dinoroseobacter shibae DFL-12

Annotation: ATP-dependent helicase HrpB (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 834 TIGR01970: ATP-dependent helicase HrpB" amino acids 27 to 832 (806 residues), 893.1 bits, see alignment E=1e-272 PF00270: DEAD" amino acids 42 to 185 (144 residues), 34.6 bits, see alignment E=3.2e-12 PF00271: Helicase_C" amino acids 228 to 351 (124 residues), 40 bits, see alignment E=8.3e-14 PF08482: HrpB_C" amino acids 689 to 820 (132 residues), 154.5 bits, see alignment E=4e-49

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 100% identity to dsh:Dshi_2580)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LHX9 at UniProt or InterPro

Protein Sequence (834 amino acids)

>Dshi_2580 ATP-dependent helicase HrpB (RefSeq) (Dinoroseobacter shibae DFL-12)
MLRPWREYVRNNEAVGVTSGGGKSASLPIDGALPELLAAMAGSGMAVLQAPPGAGKTTRV
PLALLEAGAAGRIVMLEPRRLAARAAAERMAASLGEPVGATVGYRMRGASKVGPATRIEV
VTEGILTRWLMADPELPGIGTVIFDEFHERSLNADLGLALCLEVRGALREDLNLLVMSAT
LDAEPVAALMGGAPVVTSAGRAFPVEARYLERPLPRGARFAPAVADLVLQALEEAEGSAL
VFLPGEGEIRQVAALLRDRVGRDVVVRPLFGAMKLSEQRAAIAPAPEGQRKVVLATAIAE
TSLTIEDVRIVVDGGRARRARFDPGSGMSRLVTEPVSRAEAAQRQGRAGRVAPGVCYKLW
TRGAEGAFAAYAPPEIAVADLSGLALDLAVWGTGPEDLALLTPPPEGAWAEARALCAMLG
ALDAEGRVTDHGRALARMPTHPRLAHMLHVAGRQGTELAALLSERDLDRGAGVDLARRVA
ILRGDAPAPDPGARARVREEAKRLARDLPERAALSLGAQAALAYPDRIGLRRAGEAPRYL
LAGGKGAVMEPDAPLAGMRLIVATDLDGDPREARIRQAVAVTEGEVRALFADRILWRDLC
AWSKRFRKVVARQQECLGALVLADRVWRDVPEAARAAAALEGVRDLGLAALGGGPAFERL
RARVALCRAGGLEVPDMSEAALLEAGEAWLLPVLGQVRDADDLRRIDPLEPLRAWLGWDA
LQAVEAQAPGAITTPLGRKLPVDYSGEVPEVQARVQEFYGVTRHPTVGPGRVPLRLTLLS
PGQKPVAVTSDLPGFWVGGYADLRKDMRGRYPRHPWPEDPTEAAPTTRAKPRKG