Protein Info for Dshi_2485 in Dinoroseobacter shibae DFL-12

Annotation: pyruvate carboxylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1145 PF00289: Biotin_carb_N" amino acids 4 to 113 (110 residues), 137.1 bits, see alignment E=1.3e-43 TIGR01235: pyruvate carboxylase" amino acids 6 to 1145 (1140 residues), 1794 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 118 to 326 (209 residues), 226.2 bits, see alignment E=1.3e-70 PF02222: ATP-grasp" amino acids 142 to 297 (156 residues), 27.2 bits, see alignment E=1.1e-09 PF02785: Biotin_carb_C" amino acids 345 to 453 (109 residues), 116.1 bits, see alignment E=3.4e-37 PF00682: HMGL-like" amino acids 534 to 806 (273 residues), 103.1 bits, see alignment E=7.9e-33 PF02436: PYC_OADA" amino acids 828 to 1027 (200 residues), 260.8 bits, see alignment E=3.4e-81 PF00364: Biotin_lipoyl" amino acids 1078 to 1144 (67 residues), 46.6 bits, see alignment 9.7e-16 PF13533: Biotin_lipoyl_2" amino acids 1081 to 1111 (31 residues), 25.5 bits, see alignment (E = 3.6e-09)

Best Hits

Swiss-Prot: 48% identical to PYC1_CAEEL: Pyruvate carboxylase 1 (pyc-1) from Caenorhabditis elegans

KEGG orthology group: K01958, pyruvate carboxylase [EC: 6.4.1.1] (inferred from 65% identity to amv:ACMV_29950)

MetaCyc: 48% identical to pyruvate carboxylase, mitochondrial (Homo sapiens)
Pyruvate carboxylase. [EC: 6.4.1.1]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LSM0 at UniProt or InterPro

Protein Sequence (1145 amino acids)

>Dshi_2485 pyruvate carboxylase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTDFQKILIANRGEIAIRVMRAANELGKKTVAVYAEEDKLCLHRFKADEAYKIGEGLGPV
AAYLSIDEIIRVAKLSGADAIHPGYGLLSENPDFVDACVANGIAFIGPRAETMRALGDKA
SARRVAIEAGVPVIPATEVLGDDMDKVRAEAEAIGFPLMLKASWGGGGRGMRPIFDPDEV
ADKVREGRREAEAAFGNGEGYLEKMITRARHVEVQILGDSMGNIYHLWERDCSVQRRNQK
VVERAPAPYLSSSQREQLCELGRKICAHVNYECAGTVEFLMDMDTGEFFFIEVNPRVQVE
HTVTEEVTGIDIVRAQILIAEGKSLVEATGMASQYDVQLNGHAIQCRITTEDPQNNFIPD
YGRITAYRGATGMGIRLDGGTAYSGAVITRYYDSLLEKVTAWAPTPEAAIARMDRALREF
RIRGVSTNIAFVENLLKHPTFLNNQYTTKFIDETEELFQFPKRRDRATRILRYIADISVN
GHPETEGRPKPPATPRALRAPAPKAAPAPGTRTLLEKKGPQAVADWMAAQKQLLITDTTM
RDGHQSLLATRMRSIDMIRVAPSYAANLPQLFSVECWGGATFDVAYRFLQECPWQRLRDI
RAAMPNVMTQMLLRGSNGVGYTNYPDNVVRAFTLQAAESGVDVFRVFDSLNWVENMRVAM
DAVLESGKVCEGAICYTGDILDPNRAKYDLNYYVTMGKELREAGAHILGLKDMAGLLKPA
SARILIKALKEEVGLPIHFHTHDTAGIASATILAAAESGVDAVDCAMDALSGNTSQATLG
TVVEALQHTDRDTGLDIKAVREISDYWEAVRGEYAAFESGMQAPSSEVYLHEMPGGQFTN
LKAQARSLGLEERWHEVAQTYADVNQMFGDIVKVTPSSKVVGDMALMMVSQGLSRADVED
PDRDVSFPDSVIDMMRGNLGQPAGGFPEAIVAKILKGESPNTERPGKHIPPVDLEKTRAD
LAAQLEVDIDDEDLNGYLMYPKVFTDYMTRHAEYGPVRTLPTRTFFYGMQPGDEIEVEID
PGKTLVVRMQTASETNEDGEVKVFFELNGQPRQVRVPNRKAAASVAKRPKAELGNPNHVG
APMPGVVASVAVQAGASVKEGDLLLTIEAMKMETGIHAERDAVVKAVHVTPAAQIDAKDL
LVELE