Protein Info for Dshi_2402 in Dinoroseobacter shibae DFL-12

Annotation: glycyl-tRNA synthetase, beta subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 720 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 3 to 718 (716 residues), 563.5 bits, see alignment E=5e-173 PF02092: tRNA_synt_2f" amino acids 4 to 576 (573 residues), 618.5 bits, see alignment E=1.3e-189 PF05746: DALR_1" amino acids 608 to 708 (101 residues), 38.6 bits, see alignment E=1e-13

Best Hits

Swiss-Prot: 100% identical to SYGB_DINSH: Glycine--tRNA ligase beta subunit (glyS) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to dsh:Dshi_2402)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LS45 at UniProt or InterPro

Protein Sequence (720 amino acids)

>Dshi_2402 glycyl-tRNA synthetase, beta subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MPELLIELFSEEIPARMQARAAQDLQKLVTNGLVEAGITYAGARAYATPRRLTLVVSDML
AASAPVREERKGPKADAPEKAIEGFLRSTGLGREQLETRETPKGDVLFAVIEKPGRPAAE
IVAEVLETTIRNFPWPKSMRWGSSSLRWVRPLHRILCLIRDEEGAEIVPLTVDGITAGDV
TEGHRFLAPGEIKVHGFEDYETKLRRAKVILDAEERAEHIWADATNQAFAQGLEVVEDRR
LLSEVAGLVEFPVPLMGEIDADFLDLPPEVLQTSMKEHQKFFSVRDLKSGKIVRFVTVAN
TETTDHGATILAGNRKVLSARLADAKFFWENDLRIARAGMQPWREALENVTFHNKLGSQA
ARIARIADLAATLAPQVGADPDAARTAAELAKADLSSEMVFEFPELQGLMGRYYAEAAGH
PAEIAAVAQDHYQPLGPSDAVPTAPLSIAVALADKLDTLTGFWAIDEKPTGSKDPFALRR
AALGVIRIVLENGLRLPLINSVKHHNSENTSRPLFSALVAHAKTDQERADGSKSMSDWKA
LVARSQDDLLSFFHDRLKVFLRDEGIRHDIIDACLAMEGNDDLLLLVNRARALSETLKTE
DGENLLQGFKRANNILTQAEEADGVEYSYGADPKFAETDEERALFAALDTAEAVIDPALE
AEDFAAATAAMASLRAPLDAFFEAVQVNAENQILRRNRLNLLSRIRATCLKLADLTRIEG