Protein Info for Dshi_2401 in Dinoroseobacter shibae DFL-12

Annotation: PEP-utilizing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00391: PEP-utilizers
  • amino acids 390 to 472 (83 residues), 88 bits, E=2.7e-29
• PF02896: PEP-utilizers_C
  • amino acids 488 to 837 (350 residues), 255.1 bits, E=8.1e-80

Best Hits

KEGG orthology group: K01006, pyruvate,orthophosphate dikinase [EC: 2.7.9.1] (inferred from 100% identity to dsh:Dshi_2401)

Predicted SEED Role

"Pyruvate,phosphate dikinase (EC 2.7.9.1)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.1)

MetaCyc Pathways

• nitrogen remobilization from senescing leaves (6/8 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (5/11 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.1

Sequence Analysis Tools

See A8LS44 at UniProt or InterPro

Protein Sequence (862 amino acids)

>Dshi_2401 PEP-utilizing protein (RefSeq) (Dinoroseobacter shibae DFL-12)
MQKNAPFTEITRTVDLSAETHGQRAKCIQRLIRLDMPVPHTVALSFETVHGIAAGQVIDT
RPLLGSFGADSLVSVRPSPAMQEWGGPTAMLDVGMNDTMLLTLSETIGAEAARALYLRFV
QAYAVEVARLDPELFEPTEPTRDALVQSLEIYEEEMDEPFPQDPANQLAAVLKSMARAWE
GTSARLLRQAKGAPPDAGLGLVVQRMALGMGAAESGSGVIQFVSSETGLAQITGRYLSQS
QGRDALARRAEALYLSRDSRGPSLEDACPEAFAELRAFGDVLRTGLREEMQIEFALDAGK
LSVLDAVRVPRAPRAAVRIAVALAESGVISKEDAILRIEPRTLPELLHRQVAFDAQRDPI
AKGMAASPGAATGQLVFSATAAQACAAQETPCILVRPETSPEDIRGMHAASGVLTEKGGM
TSHAAVIARGLGKPCVVGTRDITLDLRAKTLTTRDGRVFAEGDTVTLDGTAGACLAGAPR
LMEPALDGSFRTLMAWADQARDIGIRANADTPADAETARRFDAEGIGLCRTEHMFFDEDR
LTVMHEMIFAETGEARGAALERLLPMQREDFVDLFEIMRGKPVCIRLFDPPLHEFLPTSR
EGIWNLAEALDLPVSTVTHRIEALSEYNPMLGMRGVRLGIAIPEIYEMQARAIFEATVDV
SLKGAIVTPEIMLPLVSARREVELLKTNIDAVAAAVRNERGTRFGFRLGVMVETPRAALK
AGEIAEITDFLSFGTNDLTQMTYGLSRDDAGRFMGTYVQQGVFAEDPFHTLDADGVGELL
NIAAERGRAVKPDLTLSICGEHGGDPDSIDFCRKAGFSYVSCSPFRVPVARMAAAHLALT
DAPVEKKPRRFNLLGRVLRSGS