Protein Info for Dshi_2365 in Dinoroseobacter shibae DFL-12

Annotation: urease, alpha subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 PF00449: Urease_alpha" amino acids 4 to 121 (118 residues), 185.8 bits, see alignment E=4.8e-59 TIGR01792: urease, alpha subunit" amino acids 5 to 569 (565 residues), 981.6 bits, see alignment E=6.7e-300 PF01979: Amidohydro_1" amino acids 127 to 449 (323 residues), 250.7 bits, see alignment E=3.8e-78 PF07969: Amidohydro_3" amino acids 376 to 449 (74 residues), 33.1 bits, see alignment E=6.7e-12

Best Hits

Swiss-Prot: 100% identical to URE1_DINSH: Urease subunit alpha (ureC) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 100% identity to dsh:Dshi_2365)

MetaCyc: 79% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LRS0 at UniProt or InterPro

Protein Sequence (569 amino acids)

>Dshi_2365 urease, alpha subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MPATISRAAYADMYGPTTGDKVRLADTDLIIEVERDLTTYGEEVKFGGGKVIRDGMGQSQ
ATRAEGAVDTVITNALIVDHTGIYKADVGLRDGVIAAIGKAGNPDTQTGVDIIIGPGTEA
IAGEGRILTAGGFDSHIHFICPQQIEDALHSGITTMIGGGTGPAHGSLATTCTPGAFHIG
RMLQSLDAFPMNFGLAGKGNASRPGALVEMIEAGACAMKLHEDWGTTPGAIDCCLSVADD
MDVQVMIHTDTLNESGFVENTIAAMKGRTIHAFHTEGAGGGHAPDIMKVVGFEHVLPSST
NPTMPYTVNTLEEHLDMLMVCHHLDKAIPEDVAFAESRIRKETIAAEDILHDMGAFSIIA
SDSQAMGRVGEVIIRTWQTADKMKKQRGRLAEETGDNDNFRVRRYIAKYTINPAIAHGIS
AHIGSIEVGKRADLCLWNPAFFGVKPEMILMGGTIVCAQMGDPNASIPTPQPVYTRPMFG
AYGRSVENSAVLFVSQAGQAAGIGASLGLAKQTLAVKTCRDIGKSSMVHNSATPEVEVHP
ETYEVRADGELLTCEPATELPLAQRYFMF