Protein Info for Dshi_2226 in Dinoroseobacter shibae DFL-12
Annotation: Glutamate--putrescine ligase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 100% identity to dsh:Dshi_2226)Predicted SEED Role
"glutamine synthetase family protein" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- ammonia assimilation cycle II (2/2 steps found)
- L-glutamine biosynthesis I (1/1 steps found)
- L-aspartate degradation II (aerobic) (1/3 steps found)
- L-aspartate degradation III (anaerobic) (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.2
Use Curated BLAST to search for 6.3.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LR39 at UniProt or InterPro
Protein Sequence (456 amino acids)
>Dshi_2226 Glutamate--putrescine ligase (RefSeq) (Dinoroseobacter shibae DFL-12) MAETPLERMLASVPEPARAYLEGRRLDEVECIVADLAGVARGKAMPAAKFARQMSFYLPN SIFFQTITGGWAEAALDGFTEPDMVLKPDFSTATPAPWTADWTLQIIHDIEDQSGAPMPV APRNVLKRVIALYKARGWKPVVAPEMEFYLTARNIDPAIAIQPPMGRTGRRAAARQAYSM SAVDEYGPVIDDIYDFAEAQGLEIDGITQEGGAGQIEINLRHGDPLKLADEVFYFKRLIR EAALRHDCFATFMAKPIQDEPGSAMHIHHSVTDIATGENIFTAPDGTETPAFQHFIAGLQ SHLGDVVPLYAPYVNSYRRYVPENAAPINLEWARDNRTAGLRVPVANPAARRVENRLAGM DCNPYLGIAASLACGYLGLLAEKPPKPQYVGDAYSGSEDLAPELGAALDLFSEATAVHDI LGPEFCRVYEIVKRHEYTEFLQVISPWEREHLLLNV