Protein Info for Dshi_2132 in Dinoroseobacter shibae DFL-12

Annotation: amidophosphoribosyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01134: amidophosphoribosyltransferase
  • amino acids 38 to 490 (453 residues), 485.7 bits, E=9e-150
• PF13522: GATase_6
  • amino acids 102 to 235 (134 residues), 74 bits, E=1.2e-24
• PF13537: GATase_7
  • amino acids 124 to 239 (116 residues), 74.2 bits, E=9.2e-25

Best Hits

Swiss-Prot: 50% identical to ASE3_ARATH: Amidophosphoribosyltransferase 3, chloroplastic (ASE3) from Arabidopsis thaliana

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 100% identity to dsh:Dshi_2132)

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

See A8LQK1 at UniProt or InterPro

Protein Sequence (508 amino acids)

>Dshi_2132 amidophosphoribosyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSHPDTPADRPPKMPPAHPFDFDAVRSDDGDDKLHEECGVFGVVGLPEAANFVALGLHAL
QHRGQEAGGIVSHAPGIGFNSARRFGYVRDNFTKASLMETLPGHLAIGHVRYSTAGSKGH
TQIRDVQPFFGEFSMGGAAIAHNGNITNADALRRELIDRGSIFQSSSDSECIIHLMARSL
QRNIPERMKDALRRCEGAFSVVAMTRTKLIGVRDPLGVRPLVLGKLGDGWALSSETCALD
IIGAEFVREIEPGEMVVITENEGVQSYFPFERRASRFCIFEHVYFSRPDSIIGGRSVYDT
RRMIGVELAKEAPVDADLVCPVPDSGTPAAIGYSQESGIPYAMGIIRNQYMGRTFIEPTE
QIRNMGVRLKLNVNRALIKGKRVILVDDSVVRGTTSRKIKEMILDAGAAEVHFRIASPPT
SWPCFYGVDTPEREKLLAATMSEDEMCAHLGVNSLRFITLDGLYRAAGVPEGRDPAAPRY
CDACFSGEYPVAPSDMLEKGFQLRPAAE