Protein Info for Dshi_2115 in Dinoroseobacter shibae DFL-12

Annotation: sarcosine oxidase, alpha subunit family (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 4 to 999 (996 residues), 1535.8 bits, see alignment E=0 PF13510: Fer2_4" amino acids 16 to 101 (86 residues), 86.9 bits, see alignment E=3.5e-28 PF12831: FAD_oxidored" amino acids 171 to 232 (62 residues), 49 bits, see alignment 2.6e-16 PF07992: Pyr_redox_2" amino acids 171 to 430 (260 residues), 33.8 bits, see alignment E=1.1e-11 PF01134: GIDA" amino acids 171 to 216 (46 residues), 22.8 bits, see alignment 2.1e-08 PF00890: FAD_binding_2" amino acids 171 to 208 (38 residues), 25.7 bits, see alignment (E = 3e-09) PF13450: NAD_binding_8" amino acids 174 to 212 (39 residues), 22.9 bits, see alignment (E = 4e-08) PF17806: SO_alpha_A3" amino acids 516 to 597 (82 residues), 114.5 bits, see alignment E=1e-36 PF01571: GCV_T" amino acids 616 to 885 (270 residues), 325.8 bits, see alignment E=7e-101 PF08669: GCV_T_C" amino acids 905 to 991 (87 residues), 72.7 bits, see alignment E=8.3e-24

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to dsh:Dshi_2115)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQI4 at UniProt or InterPro

Protein Sequence (1000 amino acids)

>Dshi_2115 sarcosine oxidase, alpha subunit family (RefSeq) (Dinoroseobacter shibae DFL-12)
MTEYRVEGRGRVNRKTPVRFTFDGQTYEGLKGDTVASALLANGVHLMGRSFKYHRPRGVV
TAGSEEPNALVGTTRGKGRFEPNTRATIQEIYPGLETESQNKWPSLKFDLGAINDRLYML
FSAGFYYKTFMWPRSFWDSVYEPLIRKAAGLGKAPTEVDPDTYASRYMHCDVLVVGSGPS
GIAAALTAGRAGSKVVLVDENAEMGGTLLSEPSVSIDGQPAWEWLEGAMAELDRMPNVRR
MTRTTAMGYYHQNMIGMVEKLTDHMAEIPEGAPRERMWRVRAREVVLAQGAIERPMVFDG
NDTPGILMAGAAQTFLNRFGVLVGKSPVVLTSHDSAWYSAFDMADAGASVVAIVDTRNDV
SQALRDEAQARGLKTYLGYTATGTKGRLRIKALRVNPVRDGKVGAGHLLFCDAVLMCGGW
TPSLHLFSHTKGSLHWDAESKAYLPGKKSEDVHIAGAGRGLWGIASALEDGAKAGAAAVQ
SLGGSAGSATYAVADDRTGTGVTQKELPTDRSPGKAKAFVDFQNDVTAKDIRLAVREGMK
SIEHVKRYTTNGMATDQGKLSNMNGLTIAADALGKEAPQVGLTTFRPPYTPTTFGAFCGY
HKGAHFEVTRKTPIDPWAEEHGAAFEPVALWRRAWYFPQAGEDMHKAVARECKSTRESVG
MFDASTLGKIEVSGPDAVEFMNRMYTNPWTKLGVGRCRYGLLLGEDGFIRDDGVIGRMRD
DLFHVTTTTGGAARVLNMMEDYLQTEWPELKVWLTSTTEEWATIALNGPNARKLLAPFVE
GADISADAFPHMSVVECTVAGFPSRLFRISFTGELGFEINVPARHGKALWEKLWEAGQQY
DICPYGTETMHVLRAEKGYIIVGQDTDGTVTPQDAGIGWAIGKAKPDFVGKRSLQRPDIV
APGRKQLVGLLTEDPKTVLAEGAQIVDDPKQAKPMKMIGHVTSSYWSETLGRSIAMAVVE
GGFDRMDSTLHIPTESGDAVPAKVTGTVFYDPAGDRLKVE