Protein Info for Dshi_2033 in Dinoroseobacter shibae DFL-12

Annotation: TRAP dicarboxylate transporter, DctM subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 transmembrane" amino acids 20 to 48 (29 residues), see Phobius details amino acids 60 to 83 (24 residues), see Phobius details amino acids 106 to 131 (26 residues), see Phobius details amino acids 149 to 176 (28 residues), see Phobius details amino acids 182 to 205 (24 residues), see Phobius details amino acids 226 to 248 (23 residues), see Phobius details amino acids 258 to 278 (21 residues), see Phobius details amino acids 290 to 315 (26 residues), see Phobius details amino acids 330 to 360 (31 residues), see Phobius details amino acids 373 to 396 (24 residues), see Phobius details amino acids 412 to 437 (26 residues), see Phobius details PF06808: DctM" amino acids 20 to 432 (413 residues), 301.9 bits, see alignment E=3.7e-94 TIGR00786: TRAP transporter, DctM subunit" amino acids 30 to 436 (407 residues), 310.1 bits, see alignment E=1e-96

Best Hits

Swiss-Prot: 37% identical to DCTM_PSEAE: C4-dicarboxylate TRAP transporter large permease protein DctM (dctM) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_2033)

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, large permease component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LPR8 at UniProt or InterPro

Protein Sequence (442 amino acids)

>Dshi_2033 TRAP dicarboxylate transporter, DctM subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MLWQQMDQTVILGWDFYGPVIMFVALVALAVPVWAAIGASAILMLVWSGALPLSLVGERL
FSGIDAFALTAVPLFILTGDALVRTGLSRKFLDVAEALTQFAKGGFGSATVLVCGMFAAI
SGSDAAGAAAVGRMTIARLVESGYPRPYACALVAAGACTGILIPPSIAYIIIGLVLGISA
STLFLAAVIPGLAILVSILVTNIIMNRLNGWEGGGNVSMPEYFSRLWTALKSGWYAFIVP
GIIFYGIFSGRMTPTEAGATAVVVTIVMGFVLGTLKLSDFPAMLVSSAKVNGVILPIIAF
SVPLAEALAIIGVPQGFVAVATGVSEEPWVLILMMLAILVAAGCVMETTPNIVILAPILL
PLAEEIGMNEIQFCIMMITALGVGFITPPLGLNLFVVSGITGESILKIAYRAVPFVFFML
LVTLMIAYIPAISTALLPDIFK