Protein Info for Dshi_1943 in Dinoroseobacter shibae DFL-12

Annotation: N-isopropylammelide isopropylaminohydrolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 PF07969: Amidohydro_3" amino acids 39 to 399 (361 residues), 199.3 bits, see alignment E=1.9e-62 PF01979: Amidohydro_1" amino acids 48 to 399 (352 residues), 38.8 bits, see alignment E=6.9e-14

Best Hits

KEGG orthology group: K01485, cytosine deaminase [EC: 3.5.4.1] (inferred from 100% identity to dsh:Dshi_1943)

MetaCyc: 44% identical to cytosine deaminase monomer (Klebsiella pneumoniae)
Cytosine deaminase. [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]

Predicted SEED Role

"Cytosine deaminase (EC 3.5.4.1)" (EC 3.5.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.1

Use Curated BLAST to search for 3.5.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LP02 at UniProt or InterPro

Protein Sequence (422 amino acids)

>Dshi_1943 N-isopropylammelide isopropylaminohydrolase (RefSeq) (Dinoroseobacter shibae DFL-12)
MVDILVKGGTLPDGTQADIAITGDRIVDVAPGIAAKAGEVIDATGDLVSPPFVDPHFHMD
ATLSYGIPRINASGTLLEGIALWGELKHETTIDAMIDRALRYCDWAVSMGLLAIRSHVDT
CDDSLKGVQAMLQLRETVKPYLDLQLVAFPQDGLYRDPTARENTLRALDMGLDVVGGIPH
FERTMADGAASVRDLCEIAADRGLPVDMHCDESDDPMSRHIETLAAETVRCGLQGRVAGS
HLTSMHSMDNYYVSKLLALVAEAGISAIPNPLINIMLQGRHDTYPKRRGLTRVREMQALG
IPVGWGQDCVRDPWYSLGTADMLDVAFMGLHVAQMSAPEEMARCFEMVTETNAAIIGLPD
YGLRKGALASLVVLDAADPIEAVRLRPDRLCVISKGRVVSRKARNDAALTLPGRPATVHR
RH