Protein Info for Dshi_1693 in Dinoroseobacter shibae DFL-12

Annotation: cobyrinic acid a,c-diamide synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR00379: cobyrinic acid a,c-diamide synthase" amino acids 10 to 424 (415 residues), 307.7 bits, see alignment E=7.3e-96 PF01656: CbiA" amino acids 11 to 187 (177 residues), 67.2 bits, see alignment E=2.2e-22 PF13500: AAA_26" amino acids 75 to 184 (110 residues), 44.5 bits, see alignment E=2.5e-15 PF07685: GATase_3" amino acids 242 to 426 (185 residues), 109.9 bits, see alignment E=2e-35

Best Hits

Swiss-Prot: 57% identical to COBB_SINSX: Hydrogenobyrinate a,c-diamide synthase (cobB) from Sinorhizobium sp.

KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 100% identity to dsh:Dshi_1693)

MetaCyc: 57% identical to CobB (Pseudomonas denitrificans (nom. rej.))
Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing). [EC: 6.3.5.9]

Predicted SEED Role

"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.- or 6.3.5.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLQ7 at UniProt or InterPro

Protein Sequence (431 amino acids)

>Dshi_1693 cobyrinic acid a,c-diamide synthase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSAPRGRGLLLAAPASGAGKTTVTLGILAALTARGLAVRGAKSGPDYIDPQFHAAATGQD
CLNLDPWAMTPDMLRHLASGPDLLIVEGAMGLFDGAADGSGASADLARHLGLPVVLIVDA
ARMAQSIAPLVAGFAHHDPDVTVAGVILNRVGSPRHTDMLRTALAPLGIPVLGAVPRRAD
LATPSRHLGLVQATERADLDAFLARAGAQIGATVDLDALTACARPLPDGPTVPTALPPPG
QRIAVARDAAFGFAYPHMLQAWRAAGAEVLPFAPLADDPAPEDADAIFLPGGYPELHAGK
LAAATVFLDSLRRAAGRDTLIYGECGGYMVLGDGLVDADGIRHRMAGLLTLETSFAHRKL
HLGYRTLRPLGGPFSGPFRGHEFHYAATLRAEGPPLFAAADATGVPLGETGLRRGSVCGS
FAHIVAPDSAP