Protein Info for Dshi_1501 in Dinoroseobacter shibae DFL-12

Annotation: beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01750: beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ
  • amino acids 17 to 156 (140 residues), 172.6 bits, E=2.1e-55
• PF22817: ApeP-like
  • amino acids 20 to 153 (134 residues), 30.4 bits, E=4.2e-11
• PF07977: FabA
  • amino acids 24 to 150 (127 residues), 108.3 bits, E=3.8e-35
• PF22818: ApeI-like
  • amino acids 49 to 128 (80 residues), 58 bits, E=1.2e-19

Best Hits

Swiss-Prot: 100% identical to FABZ_DINSH: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ (fabZ) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K02372, 3R-hydroxymyristoyl ACP dehydrase [EC: 4.2.1.-] (inferred from 100% identity to dsh:Dshi_1501)

MetaCyc: 43% identical to 3-hydroxy-acyl-[acyl-carrier-protein] dehydratase (Escherichia coli K-12 substr. MG1655)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase. [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]; 4.2.1.59 [EC: 4.2.1.59]

Predicted SEED Role

"3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabZ form (EC 4.2.1.59)" (EC 4.2.1.59)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• 8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (3/5 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• biotin biosynthesis I (8/15 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (21/205 steps found)
• superpathway of mycolate biosynthesis (22/239 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Fatty acid biosynthesis
• Limonene and pinene degradation
• Nucleotide sugars metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Porphyrin and chlorophyll metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-, 4.2.1.59

Use Curated BLAST to search for 4.2.1.- or 4.2.1.59

Sequence Analysis Tools

See A8LK44 at UniProt or InterPro

Protein Sequence (163 amino acids)

>Dshi_1501 beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ (RefSeq) (Dinoroseobacter shibae DFL-12)
MNAKDNETAEFETTADLAMIQRILPHRYPFLLVDKVIDMVRFKSALGIKNVTFNEPHFQG
HFPGEPIMPGVMIVEALAQTSAVLVGHSMASADEDVSVYFMSIDNCKFRRKVVPGDVMSL
RVETMRGKPGGKVWKFLGEAIVEDTVAAQAEFTAMISFPQAAA