Protein Info for Dshi_1399 in Dinoroseobacter shibae DFL-12

Annotation: acetate--CoA ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 23 to 646 (624 residues), 1010.5 bits, E=0
• PF16177: ACAS_N
  • amino acids 26 to 83 (58 residues), 77.1 bits, 1.1e-25
• PF00501: AMP-binding
  • amino acids 85 to 472 (388 residues), 294.7 bits, E=1.5e-91
• PF13193: AMP-binding_C
  • amino acids 534 to 612 (79 residues), 89.9 bits, E=2.5e-29

Best Hits

Swiss-Prot: 74% identical to ACSA_RHOCB: Acetyl-coenzyme A synthetase (acsA) from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to dsh:Dshi_1399)

MetaCyc: 58% identical to acetate--CoA ligase (Syntrophus aciditrophicus)
Acetate--CoA ligase. [EC: 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• chitin deacetylation (2/4 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1

Sequence Analysis Tools

See A8LJB9 at UniProt or InterPro

Protein Sequence (653 amino acids)

>Dshi_1399 acetate--CoA ligase (RefSeq) (Dinoroseobacter shibae DFL-12)
MLDNKDGVYTPSADFVKNAHIDSSKYSEMYAASVSDPEGFWGEHGRSLDWIKPYTKVKDT
SFAHDDVHVRWFEDGTLNVAANCIDRHLATRGDQTAIIFEPDDPAEPAQHITYRELHRQV
CKLSNVLEDLGVRKGDRVILYMPMIPEAAYAMLACARIGAIHSIVFAGFSPDALSARING
ADARLLITADYAPRGGRQTPLKSNADAALLHTRDDVKMLVVKRTGGQTTWVDGRDFDYHE
LMLEADEVQHPVEVEAEHPLFILYTSGSTGQPKGVVHSSGGYLLYAAMTQKYVFDVHEGD
VYWCTADVGWVTGHSYIVYGPLANGGTTVMFEGVPTYPDASRFWQVCDKHKVTQFYTAPT
AIRALMAKGPEFVETCDLSSLRVLGTVGEPINPEAWNWYNDLVGKGRCPIVDTWWQTETG
GHLLTPLPGAIATKPGSATLPFFGVQPVVLDPHSGAEITETECEGVLCLKDSWPGQMRTV
YGDHERFVKTYFSDYKGYYFSGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESALV
AHAKVAEAAVVGYPHDIKGQGIYCYVTLMNGEEPSEELRKELRTWVRTEIGPIAAPDLIQ
WAPGLPKTRSGKIMRRILRKIAEDDFGALGDTSTLADPSVVEDLIENRMNKKG