Protein Info for Dshi_1336 in Dinoroseobacter shibae DFL-12

Annotation: peptide chain release factor 3 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 TIGR00503: peptide chain release factor 3" amino acids 14 to 532 (519 residues), 638.1 bits, see alignment E=9.9e-196 PF00009: GTP_EFTU" amino acids 17 to 280 (264 residues), 168.1 bits, see alignment E=3.5e-53 TIGR00231: small GTP-binding protein domain" amino acids 21 to 155 (135 residues), 73.2 bits, see alignment E=2.1e-24 PF01926: MMR_HSR1" amino acids 22 to 148 (127 residues), 24.5 bits, see alignment E=5e-09 PF03144: GTP_EFTU_D2" amino acids 319 to 385 (67 residues), 38.5 bits, see alignment E=2.6e-13 PF16658: RF3_C" amino acids 394 to 518 (125 residues), 136.9 bits, see alignment E=7.5e-44

Best Hits

Swiss-Prot: 51% identical to RF3_HYDCU: Peptide chain release factor 3 (prfC) from Hydrogenovibrio crunogenus (strain XCL-2)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 100% identity to dsh:Dshi_1336)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LIW4 at UniProt or InterPro

Protein Sequence (535 amino acids)

>Dshi_1336 peptide chain release factor 3 (RefSeq) (Dinoroseobacter shibae DFL-12)
MLDRTHNAPDLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGEARRT
RSDFLAMEKDRGISVSASAMSFDYKDYRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDG
AKGVESQTQKLFEVCRLRDLPILTFCNKMDRESRDTFDIIDEIQENLAIDVTPASWPIGV
GRDFLGCYDLIHDRLELMDRADRNKVAESIEINGLDDPKLAEHVPAHLLEKLLEEVEMAR
ELLPPLDQQALRDGTLTPIWFGSAINSFGVKELMDGIGEYGPEPQVQPAEPRQVSPDEKA
VTGFVFKVQANMDPKHRDRVAFVRMVSGHFKRGMKLTHVRSKKAMSITNPVLFLASDREL
AEEAWAGDIIGIPNHGQLRIGDALTEGESIRISGIPSFAPELLQSARAGDPMKAKHLEKA
LMQFAEEGAAKVFKPAIGSGFIVGVVGQLQFEVLASRIELEYGLPVRFEPSQFTSARWIT
GEKIALDKLEASNKQHIAHDHDGDPVFLTRLQWDIDRVERDYPDLSLTATKEMMV