Protein Info for Dshi_1227 in Dinoroseobacter shibae DFL-12

Annotation: malate synthase G (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 3 to 714 (712 residues), 1073.7 bits, E=0
• PF20656: MS_N
  • amino acids 15 to 70 (56 residues), 48.6 bits, 9e-17
• PF20658: MSG_insertion
  • amino acids 156 to 230 (75 residues), 71.6 bits, E=1e-23
• PF01274: MS_TIM-barrel
  • amino acids 332 to 573 (242 residues), 212.2 bits, E=1.3e-66
• PF20659: MS_C
  • amino acids 585 to 678 (94 residues), 78.9 bits, E=8.8e-26

Best Hits

Swiss-Prot: 66% identical to MASZ_CHESB: Malate synthase G (glcB) from Chelativorans sp. (strain BNC1)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 100% identity to dsh:Dshi_1227)

MetaCyc: 60% identical to malate synthase G (Mycobacterium tuberculosis H37Rv)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• glyoxylate cycle (6/6 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (4/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (2/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (7/14 steps found)
• superpathway of pentose and pentitol degradation (15/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See A8LIB5 at UniProt or InterPro

Protein Sequence (724 amino acids)

>Dshi_1227 malate synthase G (RefSeq) (Dinoroseobacter shibae DFL-12)
MKRVERAGLQVAPELVEFLETQALPGTGVTAAAFWEGLSALVHEEGPTNRALLAKRTELQ
RKIDAWHVARKGQPHDAAAYAAFLREIGYLVPEPEDFEIDTANVDPEIATIPGPQLVVPI
TNARYALNAANARWGSLYDALYGTDALGDLPSGKGFDPERGARVMAWARDHLDHAAPIAG
VSWADVTRIDVADGALELVAGDLRPLVDPAQFAGYTTGPAGDVTSVLLRKNGLHLRVMID
PTSRIGAADPAGISDVIIEAAISAIMDCEDSVAAVDAEDKVLAYGNWLGLMKGDLSQEVT
KGGKSFTRALARDVVATAPNGDRLVLKGRALMLVRNVGHLMTNPAILDRDGAEIGEGLMD
AMVTTLIAMHDLGRAGGNSVHGSVYVVKPKMHGPEEVAFTDRIFTRVEAALGLPANTVKI
GIMDEERRTSANLKACIHAARSRVAFINTGFLDRTGDEIHTSMEAGPVLPKGEMKGTAWI
AAYEDRNVDIGLACGLKGRAQIGKGMWAMPDLMADMLEAKIGHPMSGANCAWVPSPTAAT
LHATHYHKVDVFARQDEIAAGGARGTLEDLLTIPLLEGRSLSAAEIAAEVENNAQGILGY
VVRWIDQGVGCSKVPDIHDVGLMEDRATCRISSQALANWLHHGIVSEEQVMGALRKMAAV
VDQQNAGDPAYRPMAPGFDGIAFAAACDLVFAGREQPSGYTEPVLHARRLELKAALVAPE
ALRA