Protein Info for Dshi_1212 in Dinoroseobacter shibae DFL-12

Annotation: carbon-monoxide dehydrogenase, large subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 806 TIGR02416: carbon-monoxide dehydrogenase, large subunit" amino acids 27 to 797 (771 residues), 1385.2 bits, see alignment E=0 PF01315: Ald_Xan_dh_C" amino acids 33 to 142 (110 residues), 130.6 bits, see alignment E=4.6e-42 PF02738: MoCoBD_1" amino acids 173 to 419 (247 residues), 228.1 bits, see alignment E=1.4e-71 PF20256: MoCoBD_2" amino acids 446 to 737 (292 residues), 266.3 bits, see alignment E=5.8e-83

Best Hits

Swiss-Prot: 71% identical to DCML_OLICO: Carbon monoxide dehydrogenase large chain (coxL) from Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)

KEGG orthology group: K03520, carbon-monoxide dehydrogenase large subunit [EC: 1.2.99.2] (inferred from 100% identity to dsh:Dshi_1212)

MetaCyc: 71% identical to carbon monoxide dehydrogenase large chain (Afipia carboxidovorans)

Predicted SEED Role

"Carbon monoxide dehydrogenase form I, large chain (EC 1.2.99.2)" (EC 1.2.99.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.2.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LIA0 at UniProt or InterPro

Protein Sequence (806 amino acids)

>Dshi_1212 carbon-monoxide dehydrogenase, large subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MNDMTPPTREERVEKLKGLGSSRKRVEDARFTQGKGNYVDDIKLPGMLHGDFVRSPYAHA
RVVSINAEAALALPGVVAVLTAKDLEPLSLHWMPTLAGDKQMVLADGKVLFQGQEVAFVV
AEDRYIAADAVELVEVEYEELPVLVDPFEALQSDVVLREDLEPGADGAHGPRRHHNHIFL
WEEGDKAATEQVIENAEVVVEEMVYYHRTHPCPLETCGSVASMDKVNGKLTLWGTFQAPH
VVRTVASLLSGIEEHNIRVISPDIGGGFGNKVGVYPGYVCSIVASIVTGKPVKFIEDRMD
NLMATAFARDYWMKGRISATKEGKITGLHCHVTADHGAFDACADPTKFPAGFFHICTGSY
DIPVAYVGVDGVYTNKAPGGVAYRCSFRVTEAAYFIERMIEVLAMELNMDAAELRRINFI
RKEQFPYTSALGWEYDSGDYHTAWDKALEAVDYKGLRAEQAERVEAFKRGETRKLLGIGL
THFTEIVGAGPVKNCDILGMGMFDSCEIRIHPTGSAVARLGTISQGQGHATTFAQILASE
TGIPADSITIEEGDTDTAPYGLGTYGSRSTPVAGAATALAGRKIRAKAQMIAAYLLEVHD
NDVEWDVDRFVVKGAPERFKTIQEIAYAAYNQAIPGVEPGLEAVSYYDPPNMTYPFGAYI
CVMEIDVDTGEHEIRQFYALDDCGTRINPMVIEGQVHGGLTEALAIAMGQEIAYDDIGNC
KTGTLMDFFIPTAWETPNYTTDFTETPSPHHPIGAKGVGESPNVGGVPAFSNAVHDAFRA
FGLRQSHMPHDHWRIWKTANQLGLHG