Protein Info for Dshi_1197 in Dinoroseobacter shibae DFL-12

Annotation: integral membrane sensor signal transduction histidine kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 622 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 303 to 324 (22 residues), see Phobius details PF00512: HisKA" amino acids 393 to 458 (66 residues), 35.1 bits, see alignment E=1.1e-12 PF02518: HATPase_c" amino acids 505 to 611 (107 residues), 83.7 bits, see alignment E=1.3e-27

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to dsh:Dshi_1197)

Predicted SEED Role

"Two component sensor kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LI85 at UniProt or InterPro

Protein Sequence (622 amino acids)

>Dshi_1197 integral membrane sensor signal transduction histidine kinase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSDALAPPPAASPRLIPALAVAVLLSGLVAVAAFPRIERYFLTEAAERAAATLSLTVEGL
NGALRRYAPLPALVAERPDLAALLRQPENTALQARINEDLRQTAYAIGASDVYLMDISGL
TLAASSYRKTLSFVGRSFSYRPYFTQALDGGLGRYFALGTTSGERGYFYAAPVEDNARIT
GVVAVKFTVDGFEETWRAGPATVLVRDLQGVVFMSSRPDWHFRALAPLTDAARARIEAAR
QYPLSEVTHLPTEITPLSEGAELWSIATPRGEAESYVARTARISDAGWDVTILLPTAPAR
QQALQMMAIAMLLTLLLVMAAAIYQQRRARLLERMETQRAAQAELEARVTQRTADLNLAN
RQLQQEVVERRQTEQRLRKTQAELVQAGKLAALGQMSAALSHEFNQPLAAVKSYAENAMT
FLDRDRVPEARENVTRISAMTDRMASISKHLRNFARRPQEEVGAVRLDAVIEDALSVMQV
RLKQTGAAVDYTPPEGPVWVRGGHVRLQQVVVNLLSNALDAMTHTPAPRVTLRIIARDGA
HMLEVRDIGPGIAPEAAETMFDPFFTTKAPGGGLGLGLSISYNIVRDFDGTLEAENAPGG
GAVFRLVLPAAAASRAAGTAAG