Protein Info for Dshi_1188 in Dinoroseobacter shibae DFL-12

Annotation: Organic solvent tolerance protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF04453: LptD" amino acids 282 to 642 (361 residues), 135.6 bits, see alignment E=1.4e-43

Best Hits

KEGG orthology group: K04744, LPS-assembly protein (inferred from 100% identity to dsh:Dshi_1188)

Predicted SEED Role

"Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LI76 at UniProt or InterPro

Protein Sequence (716 amino acids)

>Dshi_1188 Organic solvent tolerance protein (RefSeq) (Dinoroseobacter shibae DFL-12)
MNRVALTRRLAAALAVWLALACWTGPVRAQEAQAPPAPATLVADRIVANPDGTLIAEGAV
EIFYDGRSLKAERLTYDRLADTLEITGPIQLSSGPGFVLIASQAELGTDLQEGILQSARL
VLDRQVQIAAVEIQRVNGRYTQLYNTVASSCEVCADRSVPLWQIRARRIVHDALERQIYF
ERAVFEVVGIPVLYLPQMRVPDPTLERATGFLFPNFRTTSALGVGVEIPYFIALGPDRDL
TLSPQITTKDSRTLGLRYRQAFSRGNLTFEGAYTRDDLEEGDRGFATLFGAFDLGRDFVL
SFDLETVSDDQYYRDYGFDDEDRIDSEIEISRTRRDALDRATASYFTTFREDEDNDTIPR
FVLDGEITRRFDTPLIGGYAETSLSWLGLERPSDANEIGRDTRRLSLLGTWQRSWANDWG
MVMTATGELAIDQFHVSQDTSFPDQQTRVTPTLAAELRWPFEKSNGATRTVIEPVAQLVW
SRVSDVDVPNEDSTVAEFDEASLFDLNRFAGRDQVEGGWRANFGVGWTRFDASGWTTAVT
VGRVLREDDNTALAPGASATERASDWLTTVQLSSPTGLALLNRAQFSSDLSINKNVLRMG
WEDPLSALALSYTWLRASEAESRPADTNEFSVTGRRRFNDTWAGGLEFSYDFDASQAREA
DLSLEYRNECVLVELSVSRDFDTSLDLRSTTDFGITVSLLGFGRGDGAARTSRCGG