Protein Info for Dshi_0830 in Dinoroseobacter shibae DFL-12

Updated annotation (from data): Adenosine deaminase (EC 3.5.4.4)
Rationale: Specifically important for utilizing Adenosine. Automated validation from mutant phenotype: the predicted function (ADENODEAMIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: adenosine deaminase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01430: adenosine deaminase
  • amino acids 4 to 321 (318 residues), 296.1 bits, E=1.4e-92
• PF00962: A_deaminase
  • amino acids 5 to 323 (319 residues), 275.6 bits, E=2.8e-86

Best Hits

Swiss-Prot: 46% identical to ADE_CHESB: Adenine deaminase (Meso_3659) from Chelativorans sp. (strain BNC1)

KEGG orthology group: K01488, adenosine deaminase [EC: 3.5.4.4] (inferred from 100% identity to dsh:Dshi_0830)

Predicted SEED Role

"Adenosine deaminase (EC 3.5.4.4)" in subsystem Purine conversions (EC 3.5.4.4)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• superpathway of purine deoxyribonucleosides degradation (6/7 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• adenine and adenosine salvage V (2/3 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.4

Sequence Analysis Tools

See A8LRC5 at UniProt or InterPro

Protein Sequence (331 amino acids)

>Dshi_0830 Adenosine deaminase (EC 3.5.4.4) (Dinoroseobacter shibae DFL-12)
MKELPKLELHLHLEGAAPPAFIRGLAAEKSVNLSGIFDARGAYAYRDFVDFLRVYEAACT
VLQTPQDFYRLTRAVLEASSAEGVIYTEAFLSPDFCGGRDLDAWRDYLAAMTEAAADAEA
ADGIVMRGIVTCIRHFGPEAARETARCAAETAGAFITGFGIAGDEMKGAPKDFAYAFDMA
REAELQLTAHAGEWGGARSVRDAVRDLGVTRIGHGVQAIEDPALLEELAEAGITLEVCPG
SNVALGVYPNWRAHPIERLRKAGVPVTVSTDDPPFFHTTMSGEYENLAQSFGWELPDFAA
ITYAALDAAFCDTATREALRTRLAPTFGEFT