Protein Info for Dshi_0809 in Dinoroseobacter shibae DFL-12

Annotation: GTP-binding protein LepA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 6 to 184 (179 residues), 177.9 bits, E=6e-56
• TIGR01393: elongation factor 4
  • amino acids 6 to 598 (593 residues), 960.5 bits, E=3e-293
• TIGR00231: small GTP-binding protein domain
  • amino acids 8 to 177 (170 residues), 63.5 bits, E=2.1e-21
• PF03144: GTP_EFTU_D2
  • amino acids 208 to 278 (71 residues), 46.4 bits, E=1.5e-15
• PF00679: EFG_C
  • amino acids 402 to 486 (85 residues), 72.7 bits, E=7.2e-24
• PF06421: LepA_C
  • amino acids 490 to 596 (107 residues), 167.6 bits, E=2.8e-53

Best Hits

Swiss-Prot: 100% identical to LEPA_DINSH: Elongation factor 4 (lepA) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to dsh:Dshi_0809)

MetaCyc: 56% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (11/14 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (5/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (5/8 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A8LR11 at UniProt or InterPro

Protein Sequence (599 amino acids)

>Dshi_0809 GTP-binding protein LepA (RefSeq) (Dinoroseobacter shibae DFL-12)
MTELSRIRNFSIVAHIDHGKSTLADRLIQETNTVSARDMKEQLLDAMDIERERGITIKAN
TVRIDYTADDGQDYVLNLIDTPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVY
QAIDADHEIVPVLNKIDLPASDCDRVAEQIEDVIGIDASGAIRVSAKTGQGIHETLEAIV
HHLPAPKGTRDAPLKAMLVDSWYDAYLGVIVLVRIIDGTLRKGERIKMMSNGTVHHVDRI
GVFRPAMQPVDELGPGEIGFLTASIKQVRDTRVGDTITHEKKGAETALPGFKPSQPVVFC
GLFPVDSAEFEDLRDAIEKLALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIER
EYDIELITTAPSVIYHVYMRDGTMRELHNPADMPDLTHVDHLEEPRIKATILVPDDYLGD
VLKLCQDRRGIQMDLTYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYQMIGYRQ
DNLVKMQVLVNDEPVDALSTMVHRDRAEARGRAMCEKLKDLIPRHMFKIPIQAAIGGKVI
ARETLSALRKDVTAKCYGGDATRKRKLLDKQKAGKKKMRQFGKVDIPQEAFISALKMDG