Protein Info for Dshi_0659 in Dinoroseobacter shibae DFL-12
Annotation: oxygen-independent coproporphyrinogen III oxidase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to HEMN_PARDP: Oxygen-independent coproporphyrinogen III oxidase (hemN) from Paracoccus denitrificans (strain Pd 1222)
KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 100% identity to dsh:Dshi_0659)MetaCyc: 56% identical to coproporphyrinogen dehydrogenase (Cereibacter sphaeroides)
HEMN-RXN [EC: 1.3.98.3]
Predicted SEED Role
"Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.99.22)
MetaCyc Pathways
- 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (8/9 steps found)
- superpathway of bacteriochlorophyll a biosynthesis (20/26 steps found)
- heme b biosynthesis II (oxygen-independent) (3/4 steps found)
- superpathway of heme b biosynthesis from uroporphyrinogen-III (4/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.22
Use Curated BLAST to search for 1.3.98.3 or 1.3.99.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LQB9 at UniProt or InterPro
Protein Sequence (453 amino acids)
>Dshi_0659 oxygen-independent coproporphyrinogen III oxidase (RefSeq) (Dinoroseobacter shibae DFL-12) MTPKTQLARYGLFDTRVPRYTSYPTATHFSQATRPRDFTAWIQAIPPGSEISLYAHVPFC RRLCWFCACRTQGTQSDAPVRAYVDTLLAEIALLRAALPEGVRLSRIHWGGGTPTLLAPD LVTRLAEAMFALAPTTDRAEFSVEIDPNEIDAARLDALAAAGMNRASIGVQDFDPDIQKA IGREQRFEVTEAVVMDLRDRGIRSLNTDILYGLPFQTPVKITESVQKLLSLQPDRVALYG YAHVPWMARRQNLIPNEALPTPEARLDLFETARRLFRWDNYAEIGIDHFAHQGDGLAVAA AERRLHRNFQGYTDDSATVLIGLGASAISRFPQGYAQNASGTAQYQKAIRAGGFATVRGH DFAGDDAMRARMIEMIMCDFAVDGRELVRVFKVPEARITALFRAAQEQFGGMVELDSGAL SFRIPPDARPLTRMIARAFDRYEAPAGSHSVAT