Protein Info for Dshi_0547 in Dinoroseobacter shibae DFL-12

Updated annotation (from data): ABC transporter for Xylitol, periplasmic substrate-binding component
Rationale: Specific phenotype on Xylitol.
Original annotation: extracellular solute-binding protein family 1 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF01547: SBP_bac_1" amino acids 52 to 349 (298 residues), 110.8 bits, see alignment E=1.4e-35 PF13416: SBP_bac_8" amino acids 58 to 370 (313 residues), 120.4 bits, see alignment E=1.3e-38

Best Hits

KEGG orthology group: K02027, multiple sugar transport system substrate-binding protein (inferred from 100% identity to dsh:Dshi_0547)

Predicted SEED Role

"Various polyols ABC transporter, periplasmic substrate-binding protein" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LPH0 at UniProt or InterPro

Protein Sequence (447 amino acids)

>Dshi_0547 ABC transporter for Xylitol, periplasmic substrate-binding component (Dinoroseobacter shibae DFL-12)
MKTLRNLLCSAAVASVMASGAMADGHGWSLKDAAAPYAGTTVDVVFLLRPGYEAIEAMLP
AFEEETGIKVNVIKHPYENALGEQVRDFVAGGDLDVALIDLVWIGSFAENEWILPLADVQ
AKFPDAVDPDLDIDDFFPLVLNAFGGWNDTIYGLPFDNYSGLMFYNRCMLEEAGFDGPPS
TWEELKDVYGPALTKDGKYAFALQSKRNETQSADSFARMLWPFGGSFLNEEFRSNLMSEG
SQAGLKFRQELMEYMPDGIVAYDHAETVNAFSQGDVAMITEWSAFYSSVVSPETSRVADC
VEIAPEPMGPAGRKPALGGFSLAVASQADEAEQAAAYLFIQWATSKANAREYLERGGVPA
RQSVYQQDGLEEFKFVPALVESWQDGVPEFRPRFAEWPEITEIVQEWGTKMMLGEVTTEE
GAQEIGTRMEEVLDAAGYYSGEKPLAQ