Protein Info for Dshi_0541 in Dinoroseobacter shibae DFL-12

Annotation: oxygen-independent coproporphyrinogen III oxidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 53% identical to HEMN_BRADU: Oxygen-independent coproporphyrinogen III oxidase (hemN) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 100% identity to dsh:Dshi_0541)

Predicted SEED Role

"Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.99.22)

MetaCyc Pathways

3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (8/9 steps found)
superpathway of bacteriochlorophyll a biosynthesis (20/26 steps found)
heme b biosynthesis II (oxygen-independent) (3/4 steps found)
superpathway of heme b biosynthesis from uroporphyrinogen-III (4/6 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LNX9 at UniProt or InterPro

Protein Sequence (454 amino acids)

>Dshi_0541 oxygen-independent coproporphyrinogen III oxidase (RefSeq) (Dinoroseobacter shibae DFL-12)
MRNKTVDEIVEKYARVATPRYTSYPTAPHFEPAFPEVTYRGWLSALDASEPISLYVHIPF
CREMCWYCGCNMKLVKREGPLAEYVETLLKEIALVRAAMPGRVPVAHLHWGGGTPTALSP
DQIARIMDALRASFDILPDAEIAIESDPRTLTEPMVARLAELGFNRASFGVQEFDPKVQR
AINRIQPPEMVAKAVGMFRRHGIAAVNFDLIYGLPYQSVETLLHTVDLVSEMGPDRIALF
GYAHVPWVAKAQRMIPEESLPDARARAAQASAAARALTEAGYRAIGIDHFAKPEDGLARA
QAEGRLYRNFQGYTDDPAATLVGLGATSIGRTPQGYIQNQPETRAWARAIENGALPVAKG
RAFAGEDLMRAAVIERIMCDGYVDPDAIGARYGVPAGWWGPERDSLRDMERDGLVQCGAT
GLRVTSKGAPLARVVAAAFDSYFAASKARHSVAL