Protein Info for Dshi_0476 in Dinoroseobacter shibae DFL-12

Annotation: Pyrrolo-quinoline quinone (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 17 to 557 (541 residues), 758.8 bits, see alignment E=1.5e-232 PF13360: PQQ_2" amino acids 63 to 213 (151 residues), 50.6 bits, see alignment E=3.3e-17 PF01011: PQQ" amino acids 84 to 121 (38 residues), 21.4 bits, see alignment 2.4e-08 amino acids 137 to 168 (32 residues), 33.5 bits, see alignment (E = 3.6e-12) PF13570: PQQ_3" amino acids 481 to 521 (41 residues), 28.2 bits, see alignment 3e-10

Best Hits

Swiss-Prot: 78% identical to XOXF_PARDE: Putative dehydrogenase XoxF (xoxF) from Paracoccus denitrificans

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_0476)

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LNR6 at UniProt or InterPro

Protein Sequence (600 amino acids)

>Dshi_0476 Pyrrolo-quinoline quinone (RefSeq) (Dinoroseobacter shibae DFL-12)
MRRFSLLTSGIALCAATVAYANDDLITQMDDSKQWAIQTGDYKNHRYSELDEINAENVGD
LQVAWTFSTGVLRGHEGSPLVIGDTMYVHTPFPNIVYALDLSNEGKIIWKYEPKQDPDVI
PVMCCDTVNRGVAYADGKIFLHQADTKVVALDANTGDVLWEVQNGDPSIGETNTATVLPV
KDKVIVGISGGEFGVRGHVTAYDINSGELVWRAYSLGPDDDMLVDPENTTHLGKPIGENS
SLNSWEGDQWMIGGGTTWGWYAADMEENLFYYGTGNPSTWNPAQRPGDNRWTMTVMARDI
DTGMAKWFYQMTPHDEWDYDGVNENILTEQEFDGEMRKLLTRFDRNGLAYTMDRVTGELL
VAEKFDPVVNWTTGVDMDPNSETYGRPQVVAQYSTAQNGEDVNTTGICPAALGTKDQQPA
AYSPKTQVFYVPTNHVCMDYEPFRVSYTAGQPYVGATLSMYPAPDSHGGMGNFIAWDNIN
GEILWSIPEKFSVWSGALATAGDVVFYGTLEGHLKAVHADTGEELYRFKTPSGIIGNVMT
YEHDGRQYVGVLSGVGGWAGIGLAAGLTNPNDGLGAVGGYAALSDYTALGGQLTVFALPN