Protein Info for Dshi_0433 in Dinoroseobacter shibae DFL-12
Annotation: ornithine carbamoyltransferase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to OTC_VIBPA: Ornithine carbamoyltransferase (argF) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
KEGG orthology group: K00611, ornithine carbamoyltransferase [EC: 2.1.3.3] (inferred from 100% identity to dsh:Dshi_0433)MetaCyc: 63% identical to ornithine carbamoyltransferase ArgI (Escherichia coli K-12 substr. MG1655)
Ornithine carbamoyltransferase. [EC: 2.1.3.3]
Predicted SEED Role
"Ornithine carbamoyltransferase (EC 2.1.3.3)" in subsystem Arginine Biosynthesis extended or Arginine Deiminase Pathway or Arginine and Ornithine Degradation (EC 2.1.3.3)
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- superpathway of arginine and polyamine biosynthesis (14/17 steps found)
- L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
- L-citrulline degradation (3/3 steps found)
- urea cycle (4/5 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (2/6 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.3.3
Use Curated BLAST to search for 2.1.3.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LN37 at UniProt or InterPro
Protein Sequence (333 amino acids)
>Dshi_0433 ornithine carbamoyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12) MAFNLKNRHFLTLRGFTPEEIGFLLKLSADLKSAKYAGTEVPTLRGKEIALIFEKDSTRT RVGFEVAAHDQGATVTYLGPSGTHLGKKETVKDTARVLGRVYDAIEYRGFGQEIVQELAD WAGVPVYNGLTDEFHPTQILADFLTMQEHCEKPLREVAYCFMGDAGNNMGDSLLIGGAKM GMDVRLCAPRSLWPVQSIQDEARAIAEHTGARVTLTEDVDKAVKGVDFVYTDVWVSMGEP VEKWAERIDLLMPYQVNADVMARTGNPRVRFMHCLPAFHNAETEVGCDIQDKFGLEAMEV TEEVFESPASIVFDQAENRMHTIKAVLVATLGG