Protein Info for Dshi_0257 in Dinoroseobacter shibae DFL-12

Annotation: peptidase S8 and S53 subtilisin kexin sedolisin (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 PF00082: Peptidase_S8" amino acids 180 to 436 (257 residues), 172.3 bits, see alignment E=7.3e-55

Best Hits

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_0257)

Predicted SEED Role

"serine alkaline protease (subtilisin E)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLJ6 at UniProt or InterPro

Protein Sequence (449 amino acids)

>Dshi_0257 peptidase S8 and S53 subtilisin kexin sedolisin (RefSeq) (Dinoroseobacter shibae DFL-12)
MPGSGQVLNRDGHPTHIVVFEKPSENNAAMLSSMLGDRSPKGANGALRFQARGARKTSAL
LYERLAVAEMDMTEAEARDMRDQSGIAAVERNEVMSIPRPVRLDPAEAQEAEEGLRPPSR
AMGAERDAYLRGVRDLANMLLGEGPRPTGPAGAQGFWRPRNWTTWGLDAIGSDRRPELTG
EGVKVAVLDTGLDLTHPDFTDVLGARTASFVGTETAQDGHGHGTHCCGTVAGPLQPALGP
RYGVAPGAELLVGKVLADDGFGMMSQIIDGITWALNEGAQVISMSLGSARAEGAGFSRVY
EMVFRRLLSEGVLPICAAGNESDRPGLIAPVGNPAACPGAMAVAAVDRRMDPGDFSCGQV
DRIGLLDIAGPGVGVHSAWTGGGYRRISGTSMATPHVAGVAALHLQADPSTTGDDLRALM
KMQATALGPVRDFGAGLVQVPANPGVPVG