Protein Info for Dshi_0194 in Dinoroseobacter shibae DFL-12

Annotation: (p)ppGpp synthetase I, SpoT/RelA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 TIGR00691: RelA/SpoT family protein" amino acids 34 to 702 (669 residues), 685.7 bits, see alignment E=3.6e-210 PF13328: HD_4" amino acids 34 to 183 (150 residues), 165.2 bits, see alignment E=3e-52 PF01966: HD" amino acids 53 to 152 (100 residues), 37.6 bits, see alignment E=7.4e-13 PF04607: RelA_SpoT" amino acids 243 to 352 (110 residues), 144.8 bits, see alignment E=3.6e-46 PF02824: TGS" amino acids 397 to 455 (59 residues), 72.1 bits, see alignment 9.1e-24 PF19296: RelA_AH_RIS" amino acids 467 to 554 (88 residues), 63.6 bits, see alignment E=8.2e-21 amino acids 563 to 618 (56 residues), 37.9 bits, see alignment 6.2e-13 PF13291: ACT_4" amino acids 632 to 706 (75 residues), 54.7 bits, see alignment E=3.9e-18

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to dsh:Dshi_0194)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLD3 at UniProt or InterPro

Protein Sequence (714 amino acids)

>Dshi_0194 (p)ppGpp synthetase I, SpoT/RelA (RefSeq) (Dinoroseobacter shibae DFL-12)
MPRDSSDSLIEARDLIALVQTYNPRTNAALIEAAYDYGREMHRGQLRHSGEEYFTHPVAV
AAILAEQRLDDSTIITALLHDTVEDTRSTFAELESQFGREIAELVNGVTNLTNLQLSSRE
TKQAENFRKLFMAMSKDLRVILVKLADRLHNMRTIKHMPVEKQVKKARETMDIYAPLAGR
MGMQWMRDELEDLSFRVLNPDGRQSIMRRFIKLQNETGDVVQKITSDIRKELDAHGIEAD
VYGRAKKPYSIWRKMEAKQLGFSRLSDIYGFRVITHSEDDCYGVLGAIHQRWRAVPGRFK
DYISQPKSNGYRSIHTTVSGRDGKRVEVQIRTRQMHEVAEAGVAAHWSYRDGVQVENPFA
VDPAEWIASLSERFESSEDHGEFLEHVKLEMYSDQVFCFTPKGEVVKLPKGATPLDYAYG
IHTRIGDSCVGAKVDGIRVPLWTRLKNGQSVEIITADGQRPQATWIDIVVTGRAKQAIRR
SLREEDRARFVKLGQELARVAFDHVGKRVTDKALKTAAHQLGYGGRLELLSALGSADISA
RDVVEVLYPELQGQGGDEVAADRAVVGLHSDQSFERARCCQPVPGERIVGIAYRGHGVRV
HAIDCPALEEFEDEMRRWIDLQWHSGRHAAVHAVRLDVTIGNDQGVLGRICTLIGEQRAN
ISDLHFVDRKPDFYRLLLEVEVRDLEHLHRVMTAVQADTDVAGLERHRDLSRKP