Protein Info for Dshi_0175 in Dinoroseobacter shibae DFL-12

Annotation: precorrin-3B C17-methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details PF11760: CbiG_N" amino acids 49 to 128 (80 residues), 87.9 bits, see alignment E=5.5e-29 PF01890: CbiG_C" amino acids 203 to 322 (120 residues), 99.5 bits, see alignment E=2.8e-32 PF00590: TP_methylase" amino acids 339 to 548 (210 residues), 105.3 bits, see alignment E=6.6e-34 TIGR01466: precorrin-3B C17-methyltransferase" amino acids 340 to 590 (251 residues), 263.6 bits, see alignment E=8.4e-83

Best Hits

KEGG orthology group: K13541, cobalamin biosynthesis protein CbiG / precorrin-3B C17-methyltransferase [EC: 2.1.1.131] (inferred from 100% identity to dsh:Dshi_0175)

Predicted SEED Role

"Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.131

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLB4 at UniProt or InterPro

Protein Sequence (601 amino acids)

>Dshi_0175 precorrin-3B C17-methyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MAVTLVVVAISRAGEDTAHRIAAARGWAVHGRAGRVDRADAHFDNALTHVRDLFAAGVPV
VGVCAAGILIRAVAPLLADKTAEPPVVAVAEDGSVVVPLLGGHRGANRIAADLSDLLGGV
SAVTTAGDVALGVALDAPPSGYRLANPGDAKSVMAGLLSGGGARLTGEALFDLPAGEGAE
IAVTEAPLAGGPDRLVYHPQRFALGAGCARDADPQVLRAQVFEMLAEAGVAPGAVASLNT
LGLKADEPAMHALAAELGVPLRVFTAAELEAEAARLATPSEVVFAEVGCHGVAEGAALAA
AGPDAVLVQPKRKTATTTCALARAPAPITALRGRARGRVSVVGIGPGQSAWRTPEASRLV
AEAEELVGYGLYIDLLGPLAAGKARADFPLGGEEDRCRYALERAGEGRNVALVCSGDAGI
YAMGALVFELLDRGPEAQGVSDAARRVEVICAPGVSALQAAAARAGAPLGHDFCAISLSD
LLTPRADILRRLRAAAEGDFVIAFYNPVSKTRRTLLAEAREILLAHRPADTPVLLASSLG
RPEEEIRYRRLDGLEVDEVDMLTVVLVGSSNSKLAQLGAGPRMYTPRGYARKIDGDLSDA
G