Protein Info for Dshi_0144 in Dinoroseobacter shibae DFL-12

Annotation: ammonium transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 32 to 57 (26 residues), see Phobius details amino acids 77 to 96 (20 residues), see Phobius details amino acids 134 to 153 (20 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 195 to 215 (21 residues), see Phobius details amino acids 235 to 252 (18 residues), see Phobius details amino acids 272 to 293 (22 residues), see Phobius details amino acids 302 to 319 (18 residues), see Phobius details amino acids 325 to 344 (20 residues), see Phobius details amino acids 352 to 373 (22 residues), see Phobius details amino acids 380 to 404 (25 residues), see Phobius details TIGR03644: probable ammonium transporter, marine subtype" amino acids 35 to 434 (400 residues), 619.9 bits, see alignment E=1.8e-190 TIGR00836: ammonium transporter" amino acids 41 to 431 (391 residues), 369.2 bits, see alignment E=2.6e-114 PF00909: Ammonium_transp" amino acids 42 to 431 (390 residues), 369.4 bits, see alignment E=9.8e-115

Best Hits

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_0144)

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LKQ0 at UniProt or InterPro

Protein Sequence (437 amino acids)

>Dshi_0144 ammonium transporter (RefSeq) (Dinoroseobacter shibae DFL-12)
MKLTKLLPLAAAAIVLPSLGLAQDAAPSFDEIGPYIMTTLLFCMAGFLVFFMACGFAMLE
GGLVRSKNVTMQMTKNIALYSIAAVMYWLIGFNLMYPGDFNGYVGSFFVPTVLDPVGVAA
ADAALDYASIGSDFFFQLVFVAATASIVSGALAERIKLWPFLAFVVVLTGIMYPISGSWQ
WGGGWLSEMGFSDFAGSTVVHSVGGWAALAGAIILGPRLGKYKDGRVNPMPGSNLALATL
GTFILWLGWFGFNGGSQLAMGTVGDVSDVSRIFANTNMAAAAGALTALILTQVLYKKPDL
TMVLNGALAGLVSITASPLDPTLFGALWIGAVGGVIVVFTVPLLDKFKIDDVVGAIPVHL
FAGIWGTIAVVFYQEGASLVTQLVGIAAYGVFTFGVGLIVWYLLKLTMGIRVSEEDEING
LDTSELGMEAYPDFTKG