Protein Info for BT4353 in Bacteroides thetaiotaomicron VPI-5482

Annotation: valyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 879 TIGR00422: valine--tRNA ligase" amino acids 5 to 859 (855 residues), 950.8 bits, see alignment E=3.3e-290 PF00133: tRNA-synt_1" amino acids 15 to 572 (558 residues), 430.6 bits, see alignment E=1.4e-132 PF09334: tRNA-synt_1g" amino acids 41 to 188 (148 residues), 32.5 bits, see alignment E=1e-11 amino acids 355 to 415 (61 residues), 21.1 bits, see alignment 3e-08 PF13603: tRNA-synt_1_2" amino acids 237 to 300 (64 residues), 32.4 bits, see alignment 1.7e-11 PF08264: Anticodon_1" amino acids 615 to 759 (145 residues), 113.6 bits, see alignment E=1.9e-36 PF10458: Val_tRNA-synt_C" amino acids 812 to 877 (66 residues), 47.5 bits, see alignment 4.6e-16

Best Hits

Swiss-Prot: 100% identical to SYV_BACTN: Valine--tRNA ligase (valS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 100% identity to bth:BT_4353)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89ZM4 at UniProt or InterPro

Protein Sequence (879 amino acids)

>BT4353 valyl-tRNA synthetase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MELASKYNPADVEGKWYQYWLEHRLFSSKPDGREPYTIVIPPPNVTGVLHMGHMLNNTIQ
DILVRRARMEGKNACWVPGTDHASIATEAKVVNKLAAQGIKKTDLTRDEFLKHAWDWTEE
HGGIILKQLRKLGASCDWDRTAFTMDEERSKSVLKVFVDLYNKGLIYRGVRMVNWDPKAL
TALSDEEVIYKEEHGKLFYLRYKVEGDPEGRYAVVATTRPETIMGDTAMCINPNDPKNAW
LKGKKVIVPLVNRVIPVIEDDYVDIEFGTGCLKVTPAHDVNDYMLGEKYNLPSIDIFNDN
GTLSEAAGLYIGMDRFDVRKQIEKDLDAAGLLEKTEAYTNKVGYSERTNVVIEPKLSMQW
FLKMQHFADMALPPVMNDELKFYPAKYKNTYRHWMENIKDWCISRQLWWGHRIPAYFLPE
GGYVVAATPEEALAKAKEKTGNAALTMDDLRQDEDCLDTWFSSWLWPISLFDGINHPGNE
EISYYYPTSDLVTGPDIIFFWVARMIMVGYEYEGKMPFKNVYFTGIVRDKLGRKMSKSLG
NSPDPLELIDKYGADGVRMGMMLAAPAGNDILFDDALCEQGRNFCSKIWNAFRLIKGWTV
DDNIQASDAAKLAVHWFESKQNEVAAEVADLFSKYRLSEALMAVYKLFWDEFSSWYLEMI
KPAYGQGIDRTTYSATLCFLDNLLHLLHPFMPFITEELWQQMYERNAEEGESLMVSALSM
DTYVDTAFVAQFEVVKGVISNIRSIRLQKNIAQKEPLDLQVLGENPVAEFNAVIQKMCNL
SSITVVESKAEGASSFMVGTTEYAVPLGNMIDVEAEIARMEAELKHKEGFLQGVLKKLSN
EKFINNAPAAVLEMERKKQADAESIISSLKESIAALKKA