Protein Info for BT4110 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative pectinesterase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 PF13472: Lipase_GDSL_2" amino acids 10 to 171 (162 residues), 39.2 bits, see alignment E=1.6e-13 PF00657: Lipase_GDSL" amino acids 11 to 216 (206 residues), 31.5 bits, see alignment E=3.1e-11 PF01095: Pectinesterase" amino acids 239 to 505 (267 residues), 233 bits, see alignment E=4.9e-73

Best Hits

KEGG orthology group: K01051, pectinesterase [EC: 3.1.1.11] (inferred from 100% identity to bth:BT_4110)

Predicted SEED Role

"rhamnogalacturonan acetylesterase" in subsystem L-rhamnose utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A0B1 at UniProt or InterPro

Protein Sequence (536 amino acids)

>BT4110 putative pectinesterase precursor (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MANKNLYGGNPERGWCMVLPGFFSEDIRVDNHAANGRSSKSFISEGRWAKVISQVKKGDY
VFIQFGHNDEKADSARHTDPGTTFDDNLRRFVNETRVKGGIPVLFNSIVRRNFVQPEDAS
IATDARRAPGEQELPKEVNVLYDTHGAYLDSPRNVAKEMGVAFIDMNKITHDLVQGLGPA
ESKKLFMFVEPEKVPAFPKGREDNTHLNVYGARTIAGLTVDAIAKEIPELAKYVRHYDYV
VAQDGTGDFFTVQEAINAVPDFRKNVRTTILVRKGTYKEKIIIPESKINISLIGEDGVVL
TNDDFANKKNVFGENMGTSGSSSCYIYAPDFYAENITFENSAGPVGQAVACFVSADRAFF
KNCRFLGYQDTLYTYGKHSRQYYEDCYIEGTVDFIFGWSVAVFNRCHIHSKRDGYVTAPS
TDQGKKYGYVFYDCRLTADPDVAKVYLSRPWRPYAQAVFIRCELGKHILPEGWHNWGKKE
AEKTVFYAEYDSHGEGANPKARAAFSRQLKNLKGYEMETVLAGEDGWNPLKNDSVK