Protein Info for BT3950 in Bacteroides thetaiotaomicron VPI-5482

Annotation: phosphoglucomutase/phosphomannomutase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03990: phosphoglucosamine mutase
  • amino acids 4 to 457 (454 residues), 529.4 bits, E=3.7e-163
• PF02878: PGM_PMM_I
  • amino acids 8 to 142 (135 residues), 103 bits, E=2.2e-33
• PF02879: PGM_PMM_II
  • amino acids 171 to 264 (94 residues), 82.6 bits, E=5.4e-27
• PF02880: PGM_PMM_III
  • amino acids 271 to 376 (106 residues), 74.4 bits, E=1.6e-24
• PF00408: PGM_PMM_IV
  • amino acids 396 to 455 (60 residues), 38.4 bits, E=2.1e-13

Best Hits

Swiss-Prot: 31% identical to GLMM_METKA: Probable phosphoglucosamine mutase (glmM) from Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

KEGG orthology group: K01840, phosphomannomutase [EC: 5.4.2.8] (inferred from 100% identity to bth:BT_3950)

Predicted SEED Role

"Phosphoglucosamine mutase (EC 5.4.2.10)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 5.4.2.10)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• glycogen degradation I (7/8 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (3/3 steps found)
• D-galactose degradation I (Leloir pathway) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycogen biosynthesis I (from ADP-D-Glucose) (3/4 steps found)
• starch degradation III (3/4 steps found)
• starch degradation V (3/4 steps found)
• sucrose degradation IV (sucrose phosphorylase) (3/4 steps found)
• O-antigen building blocks biosynthesis (E. coli) (8/11 steps found)
• GDP-α-D-glucose biosynthesis (2/3 steps found)
• trehalose degradation V (2/3 steps found)
• glycogen degradation II (4/6 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-glucose biosynthesis (1/2 steps found)
• sucrose biosynthesis I (from photosynthesis) (6/9 steps found)
• CDP-6-deoxy-D-gulose biosynthesis (3/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (3/5 steps found)
• glucose and glucose-1-phosphate degradation (3/5 steps found)
• sucrose degradation II (sucrose synthase) (3/5 steps found)
• sucrose biosynthesis II (5/8 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• glucosylglycerol biosynthesis (2/5 steps found)
• β-(1,4)-mannan degradation (3/7 steps found)
• peptidoglycan recycling I (8/14 steps found)
• starch biosynthesis (5/10 steps found)
• chitin biosynthesis (4/9 steps found)
• glycogen biosynthesis III (from α-maltose 1-phosphate) (2/8 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (6/19 steps found)
• streptomycin biosynthesis (3/18 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (11/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.8

Use Curated BLAST to search for 5.4.2.10 or 5.4.2.8

Sequence Analysis Tools

See Q8A0S1 at UniProt or InterPro

Protein Sequence (462 amino acids)

>BT3950 phosphoglucomutase/phosphomannomutase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MTLIKSISGIRGTIGGGAGEGLNPLDIVKFTSAYATLIRKTCKAQSNKIVVGRDARISGE
MVKNVVVGTLMGMGWDVVDIDLASTPTTELAVTMEGACGGIILTASHNPKQWNALKLLNE
HGEFLNAEEGNEVLRIAEAEEFDYADVDHLGSYRKDLTYNQKHIDSVLALDLVDVEAIKK
ANFRVAIDCVNSVGGIILPELLERLGVKHVEKLYCEPTGNFQHNPEPLEKNLGDIMNLMK
GGKADVAFVVDPDVDRLAMICENGVMYGEEYTLVTVADYVLKHTPGNTVSNLSSTRALRD
VTRKYGMEYSASAVGEVNVVTKMKATNAVIGGEGNGGVIYPASHYGRDALVGIALFLSHL
AHEGKKVSELRATYPPYFIAKNRVDLIPEIDVDAILAKVKEIYKNEEINDIDGVKIDFAD
KWVHLRKSNTEPIIRVYSEASTMGAAEEIGQKIMDVINELAK