Protein Info for BT3924 in Bacteroides thetaiotaomicron VPI-5482

Annotation: ATP-dependent DNA helicase recG (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 PF17191: RecG_wedge" amino acids 8 to 159 (152 residues), 71.5 bits, see alignment E=1.6e-23 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 28 to 661 (634 residues), 721.1 bits, see alignment E=7.1e-221 PF04851: ResIII" amino acids 268 to 427 (160 residues), 46.9 bits, see alignment E=7.2e-16 PF00270: DEAD" amino acids 272 to 433 (162 residues), 94.7 bits, see alignment E=1.3e-30 PF00271: Helicase_C" amino acids 472 to 583 (112 residues), 68.4 bits, see alignment E=1.6e-22 PF19833: RecG_dom3_C" amino acids 612 to 698 (87 residues), 162.2 bits, see alignment E=6.4e-52

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to bth:BT_3924)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A0U7 at UniProt or InterPro

Protein Sequence (698 amino acids)

>BT3924 ATP-dependent DNA helicase recG (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MFDLTTRDIKFISGVGPQKAAVLNKELEIYSLYDLIYYFPYKYVDRSRIYYIHEIDGNMP
YIQLKGEILGFETIGEGRQRRLTAHFSDGTGIVDLVWFQGIKYILGKYKLHEEYIIFGKP
TVFNGRINVAHPDIDKPDDLKLSSVGLQPYYNTTEKMKRSFLNSHAIEKMMATVIQQIQE
PLPETLSPKILSDHHLMPLTEALRNIHFPTNPDSLRRAQYRLKFEELFYVQLNILCYAKD
RQRRYRGYIFERVGDVFNTFYSQNLPFQLTGAQKRVLKEIRNDVGSGRQMNRLLQGDVGS
GKTLVALMSMLLALDNGFQACMMAPTEILANQHYETIKELLFGMDIRVELLTGSIKGKKR
EAILTGLLTGDVKILIGTHAVIEDTVNFSSLGLVVIDEQHRFGVAQRARLWTKNIQPPHV
LVMTATPIPRTLAMTLYGDLDVSVIDELPPGRKPITTIHQFDNRRESMYRAVRKQIEEGR
QVYIVYPLIKESEKIDLKNLEEGYQHILEEFPGCTVAKVHGKMKSAEKDEQMQLFISGQA
QIMVATTVIEVGVNVPNASVMIIENAERFGLSQLHQLRGRVGRGAEQSYCILVTNYKLTE
DTRKRLEIMVRTNDGFEIAEADLKLRGPGDLEGTQQSGIAFDLKIANLARDGQLLQYVRS
IAEDIVDNDPSAQSPENEILWRQLKSLRKTNVNWAAIS