Protein Info for BT3644 in Bacteroides thetaiotaomicron VPI-5482

Annotation: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 15 to 86 (72 residues), 27 bits, E=7.1e-10
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 18 to 432 (415 residues), 384.6 bits, E=2.9e-119
• PF08245: Mur_ligase_M
  • amino acids 97 to 283 (187 residues), 148.8 bits, E=3e-47
• PF02875: Mur_ligase_C
  • amino acids 307 to 420 (114 residues), 60.5 bits, E=4.7e-20

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 100% identity to bth:BT_3644)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.10

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See Q8A1L7 at UniProt or InterPro

Protein Sequence (432 amino acids)

>BT3644 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKLSALYQIFLDCQLVTTDSRNCPEGSLFIALKGESFNGNAFAGKALETGCAYAVIDESE
YAIEGDQRYILVDDCLQTLQQLANYHRRQLGTRVIGITGTNGKTTTKELISAVLSQSHNI
LYTLGNLNNHIGVPSTLLRLKAEHDLAVIEMGANHPGEIKFLSEIAEPDCGIITNVGKAH
LEGFGSFEGVIKTKGELYDFLRKKEGSTVFIHHDNAYLMNIAEGLNLIPYGTEDDLYVNG
RITGNSPYLTFEWKAGKDGKSYQVQTQLIGEYNFPNALAAITIGRFFGVEDAKINEALAG
YTPQNNRSQLKKTDDNTLIIDAYNANPTSMMAALQNFRNMEVPHKMLILGDMRELGAESA
AEHQKIADYLKECAFEKVWLVGDQFAAAEHSFQTYPNVQEVIKELEADKPKGYTILIKGS
NGIKLSSVVDYL