Protein Info for BT3639 in Bacteroides thetaiotaomicron VPI-5482

Annotation: ThiF family protein, putative dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 238 transmembrane" amino acids 23 to 40 (18 residues), see Phobius details PF00899: ThiF" amino acids 13 to 237 (225 residues), 150.8 bits, see alignment E=1.9e-48

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_3639)

Predicted SEED Role

"Molybdopterin biosynthesis protein MoeB" in subsystem Molybdenum cofactor biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1M2 at UniProt or InterPro

Protein Sequence (238 amino acids)

>BT3639 ThiF family protein, putative dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MEENNWQQRTELLLGEEKMKRIRASHVLVVGLGGVGAYAAEMLCRAGVGRMTIVDADTVQ
PANMNRQLPAMHSTLGMPKAEVLAARYKDINPDIELTVLPVYLKDENIPELLDSAKFDFI
VDAIDTISPKCFLIYEAMKRHIKIVSSMGAGAKSDITQICFADLWDTYHCGLSKAVRKRL
QKMGVKRKLPVVFSTEQADPKAVLLTDDERNKKSTCGTVSYMPAVFGCYLAEYVIKRI