Protein Info for BT3549 in Bacteroides thetaiotaomicron VPI-5482
Updated annotation (from data): N-succinylcitrulline desuccinylase (EC 3.5.1.-)
Rationale: Important for fitness in most defined media, except when arginine is provided. Distantly related to succinyl-diaminopimelate desuccinylases, so this probably provides the missing N-succinylcitrulline desuccinylase activity. See PMC7311316.
Original annotation: acetylornithine deacetylase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 100% identity to bth:BT_3549)Predicted SEED Role
"Acetylornithine deacetylase (EC 3.5.1.16)" in subsystem Arginine Biosynthesis extended (EC 3.5.1.16)
MetaCyc Pathways
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
- L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
- L-ornithine biosynthesis I (5/5 steps found)
- superpathway of arginine and polyamine biosynthesis (10/17 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Caprolactam degradation
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- Lysine biosynthesis
- Nicotinate and nicotinamide metabolism
- Nucleotide sugars metabolism
- Sphingolipid metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.-
Use Curated BLAST to search for 3.5.1.- or 3.5.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8A1V9 at UniProt or InterPro
Protein Sequence (355 amino acids)
>BT3549 N-succinylcitrulline desuccinylase (EC 3.5.1.-) (Bacteroides thetaiotaomicron VPI-5482) MKYDIPTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTGRKGNNVWCLSPM FDLKKPTILLNSHIDTVKPVNGWRKDPFTPREENGKLYGLGSNDAGASVVSLLQVFLQLC RTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVGEPTEMQPAIAEKGLMVLDVT ATGKAGHAARDEGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNVVPD KCTFVVDIRSNELYSNEDLFAEIRKHIACDAKARSFRLNSSRIDEKHPFVQKAVKMGRIP FGSPTLSDQALMSFASVKIGPGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGLKL