Protein Info for BT3285 in Bacteroides thetaiotaomicron VPI-5482

Annotation: Holliday junction DNA helicase ruvB (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 PF05496: RuvB_N" amino acids 24 to 182 (159 residues), 260.2 bits, see alignment E=1.9e-81 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 28 to 330 (303 residues), 459.2 bits, see alignment E=2.8e-142 PF00004: AAA" amino acids 59 to 183 (125 residues), 69 bits, see alignment E=1.8e-22 PF17864: AAA_lid_4" amino acids 185 to 258 (74 residues), 102.3 bits, see alignment E=2.9e-33 PF05491: RuvB_C" amino acids 259 to 329 (71 residues), 90.6 bits, see alignment E=1.5e-29

Best Hits

Swiss-Prot: 100% identical to RUVB_BACTN: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 100% identity to bth:BT_3285)

MetaCyc: 61% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A2M0 at UniProt or InterPro

Protein Sequence (343 amino acids)

>BT3285 Holliday junction DNA helicase ruvB (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MEQEDFNIREHQLTSRERDFENALRPLSFEDFSGQDKVVENLRIFVKAARLRGEALDHVL
LHGPPGLGKTTLSNIIANELGVGFKVTSGPVLDKPGDLAGVLTSLEPNDVLFIDEIHRLS
PVVEEYLYSAMEDYRIDIMIDKGPSARSIQIDLNPFTLVGATTRSGLLTAPLRARFGINL
HLEYYDDDILSNIIRRSASILDVPCSVRAASEIASRSRGTPRIANALLRRVRDFAQVKGS
GSIDTEIAQFALEALNIDKYGLDEIDNKILCTIIDKFKGGPVGLTTIATALGEDAGTIEE
VYEPFLIKEGFMKRTPRGREVTELAYKHLGRSLYSSQKTLFND