Protein Info for BT3126 in Bacteroides thetaiotaomicron VPI-5482

Annotation: leucyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 944 PF00133: tRNA-synt_1" amino acids 11 to 144 (134 residues), 50.2 bits, see alignment E=3.6e-17 PF09334: tRNA-synt_1g" amino acids 35 to 153 (119 residues), 52.7 bits, see alignment E=6.2e-18 TIGR00396: leucine--tRNA ligase" amino acids 201 to 944 (744 residues), 697.3 bits, see alignment E=1.5e-213 PF13603: tRNA-synt_1_2" amino acids 276 to 441 (166 residues), 192.9 bits, see alignment E=7.9e-61 PF08264: Anticodon_1" amino acids 795 to 905 (111 residues), 73.8 bits, see alignment E=2.8e-24

Best Hits

Swiss-Prot: 100% identical to SYL_BACTN: Leucine--tRNA ligase (leuS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 100% identity to bth:BT_3126)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A329 at UniProt or InterPro

Protein Sequence (944 amino acids)

>BT3126 leucyl-tRNA synthetase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MEYNFREIEKKWQQRWVEEKTYQVTEDESKQKFYVLNMFPYPSGAGLHVGHPLGYIASDI
YARYKRLRGFNVLNPMGYDAYGLPAEQYAIQTGQHPAITTKANIDRYREQLDKIGFSFDW
SREIRTCEPEYYHWTQWAFQKMFNSYYCNDEQQARPIQELIDAFAIYGNEGLNAACSEEL
SFTAKEWKAKSEKEQQEILMNYRIAYLGETMVNWCQALGTVLANDEVIDGVSERGGFPVV
QKKMRQWCLRVSAYAQRLLDGLDTIDWTESLKETQKNWIGRSEGAEVQFKVKDSDLEFTI
FTTRADTMFGVTFMVLAPESDLVAQLTTPAQKAEVDAYLDRTKKRTERERIADRSVTGVF
SGSYAINPFTGEAVPIWISDYVLAGYGTGAIMAVPAHDSRDYAFAKHFGLEIRPLVEGCD
VSEESFDAKEGIVCNSPRPDVTPYCDLSLNGLTIKEAIEKTKQYVKEHNLGRVKVNYRLR
DAIFSRQRYWGEPFPVYYKDGMPYMIDEDCLPLELPEVDKFLPTETGEPPLGHAKEWAWD
TVNKCTVENEKIDNVTIFPLELNTMPGFAGSSAYYLRYMDPHNNKALVDPKVDEYWKNVD
LYVGGTEHATGHLIYSRFWNKFLHDVGASVVEEPFQKLVNQGMIQGRSNFVYRIKDTHTF
VSLNLKDQYEVTPLHVDVNIVSNDILDLEAFKAWRPEYAEAEFILEDGKYICGWAVEKMS
KSMFNVVNPDMIVDKYGADTLRMYEMFLGPVEQSKPWDTNGIDGVHRFIRKFWSLFYSRT
DEYLVKDEPATKEELKSLHKLIKKVTGDIEQFSYNTSVSAFMICVNELSNLKCNKKEILE
QLVITLAPFAPHVCEELWDTLGHETSVCDAAWPAYNEEYLKEDTINYTISFNGKARFNME
FDADAASDAIQAAVLADERSQKWIEGKTPKKIIVVPKKIVNVVV