Protein Info for BT3028 in Bacteroides thetaiotaomicron VPI-5482

Annotation: conserved hypothetical protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 PF04041: Glyco_hydro_130" amino acids 69 to 362 (294 residues), 77.3 bits, see alignment E=5.4e-26

Best Hits

Swiss-Prot: 72% identical to MGP_BACFN: 4-O-beta-D-mannosyl-D-glucose phosphorylase (BF0772) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: None (inferred from 100% identity to bth:BT_3028)

MetaCyc: 72% identical to 4-O-beta-D-mannosyl-D-glucose phosphorylase subunit (Bacteroides fragilis NCTC 9343)
RXN-12977 [EC: 2.4.1.281]

Predicted SEED Role

"COG2152 predicted glycoside hydrolase" in subsystem Predicted carbohydrate hydrolases

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.281

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A3C6 at UniProt or InterPro

Protein Sequence (396 amino acids)

>BT3028 conserved hypothetical protein (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNRYDNRLHILTKEYDELISRENEKILPGNGVFERYKYPILTAEHPPLEWRYDFNPETNP
YLMERFGINAVFNAGAIKFNGKYLVMARVEGHDRKSFFAIAESPNGIDNFRFWEYPVQLP
DLYPEETNVYDMRLTKHEDGWIYGIFCSESKDPDAPAGDLTSAIAAAGIIRSRDLKNWER
LPNLVSQSQQRNVVLHPEFVDGKYALYTRPQDGFIDAGSGGGISWALIDDITHAVIKKEI
VIEQRHYHTIKEVKNGEGPHPIKTPQGWLHLAHGVRACAAGLRYVLYLYMTSLDDPRKVI
AQPGGYFMAPVGEERTGDVSNVLFSNGWIADEDGTVYIYYASSDTRMHVATSTIERLIDY
CRHTPEDRLRSTTSVKSIYDIIEANKLVMSENAVVL