Protein Info for BT2955 in Bacteroides thetaiotaomicron VPI-5482
Annotation: formate acetyltransferase 2 (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 100% identity to bth:BT_2955)Predicted SEED Role
"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)
MetaCyc Pathways
- pyruvate fermentation to acetate IV (3/3 steps found)
- L-threonine degradation I (5/6 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
- reductive monocarboxylic acid cycle (2/2 steps found)
- superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
- mixed acid fermentation (12/16 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
- superpathway of N-acetylneuraminate degradation (16/22 steps found)
- superpathway of L-threonine metabolism (13/18 steps found)
- pyruvate fermentation to ethanol I (2/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.54
Use Curated BLAST to search for 2.3.1.54
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8A3J9 at UniProt or InterPro
Protein Sequence (714 amino acids)
>BT2955 formate acetyltransferase 2 (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482) MKNYEQKIANLRMRKLAQTQEKIEKEGLLDEDDYGRVVPPENLWNIIPNHPDGSFYGFDA WADNFCSLMNIHPVYIDADDAFAGRWMYFMSKMRPNKWNPDYSYDFLKENIKKYDLICGI GDDAHFAPDYEIGVKLGWNGLIKKIEHYQSMHHSEEQQHFYSLHLRVIRSVQGWIQRHID QAYRMAASATDDCSKNNLLEIAKVNESIINDAPTTLREACQWIIWYHLASRTYNRDGAGG QIDTLLAPFYEKDLAEGRIDKETAVYYLACFLLNDPVYWQLGGPDESGKDQTSPLSFLIL EAADKINTSLNLTVRVFDGLNKDLFRKSVEYLVKNKNGWPRFSGDKALVEGFMKNGFSAQ LARRRIAVGCNWMSLPGLEYTMNDLVKINMAKVFEVSYTEMMNEQKDHSTSRLWMLFNEH LAKAVQTSADGIRHHLKFQKYNEPELLLNLLSHGPLEKGKDVSDGGAEYYNLAIDGAGLA TVADSFAALEQRIELENKLSWNTLTEYLRNNFSGDGGCRIRHLLKNSEKYGAAESLGEKW AIRIKETFTELVRLQSEPDKRRIFIPGFFSWANTVGFGQSVGATPDGRLAQTPISHGANP TPGFVKDGASLSLATIIAKIQPGYGNTAPMQWELDPTFANMDNIELIMSIIRAHFDLGGT LINVNIMNKDTVLAAHKDPAGYPELVVRVTGFTAYFSMLSPQFRQLVVDRILES